A novel picornavirus-like genome from transcriptome sequencing of sugar beet cyst nematode represents a new putative genus

Jingyu Lin, Rongjian Ye, Thanuja Thekke-Veetil, Margaret E. Staton, Prakash R. Arelli, Ernest C. Bernard, Tarek Hewezi, Leslie L. Domier, M. R. Hajimorad

Research output: Contribution to journalArticle

Abstract

Analysis of transcriptome sequence data from eggs and second-stage juveniles (J2s) of sugar beet cyst nematode (SBCN, Heterodera schachtii) identified the full-length genome of a positive-sense single-stranded RNA virus, provisionally named sugar beet cyst nematode virus 1 (SBCNV1). The SBCNV1 sequence was detected in both eggs and J2s, indicating its possible vertical transmission. The 9503-nucleotide genome sequence contains a single long open reading frame, which was predicted to encode a polyprotein with conserved domains for picornaviral structural proteins proximal to its amino terminus and RNA helicase, cysteine proteinase and RNA-dependent RNA polymerase (RdRp) conserved domains proximal to its carboxyl terminus, hallmarks of viruses belonging to the order Picornavirales. Phylogenetic analysis of the predicted SBCNV1 RdRp amino acid sequence indicated that the SBCNV1 sequence is most closely related to members of the family Secoviridae, which includes genera of nematode-transmitted plant-infecting viruses. SBCNV1 represents the first fully sequenced viral genome from SBCN.

Original languageEnglish (US)
Article number001139
Pages (from-to)1418-1424
Number of pages7
JournalJournal of General Virology
Volume99
Issue number10
DOIs
StatePublished - Oct 2018

Keywords

  • Iflavirus
  • Picornavirales
  • RNA virus
  • Secoviridae
  • Sugar beet cyst nematode
  • Sugar beet cyst nematode virus 1

ASJC Scopus subject areas

  • Virology

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  • Cite this

    Lin, J., Ye, R., Thekke-Veetil, T., Staton, M. E., Arelli, P. R., Bernard, E. C., Hewezi, T., Domier, L. L., & Hajimorad, M. R. (2018). A novel picornavirus-like genome from transcriptome sequencing of sugar beet cyst nematode represents a new putative genus. Journal of General Virology, 99(10), 1418-1424. [001139]. https://doi.org/10.1099/jgv.0.001139