A novel method for low load friction testing on living cells

Jessica A. Cobb; Alison C. Dunn; Jiwoon Kwon; Malisa Sarntinoranont; W. Gregory Sawyer; Roger Tran-Son-Tay

doi:10.1007/s10529-007-9623-z

A novel method for low load friction testing on living cells

Jessica A. Cobb, Alison C. Dunn, Jiwoon Kwon, Malisa Sarntinoranont, W. Gregory Sawyer, Roger Tran-Son-Tay

Research output: Contribution to journal › Article › peer-review

Abstract

A low load tribology technique for studying the effects of friction on living cells was developed. Results show a direct relationship between the coefficient of friction (COF) and the extent of cell damage. The COF, μ, for a glass pin on an intact layer of human corneal epithelial cells is determined to be on the order of μ = 0.05 ± 0.02 (n = 16). The correlations between applied normal load and extent of cell damage, as well as between number of reciprocation cycles and cell damage, are reported. It is also found that cell damage can occur when a loading force as low as 0.5 mN is applied, although the cells appear to be intact.

Original language	English (US)
Pages (from-to)	801-806
Number of pages	6
Journal	Biotechnology Letters
Volume	30
Issue number	5
DOIs	https://doi.org/10.1007/s10529-007-9623-z
State	Published - May 2008
Externally published	Yes

Keywords

Cell
Friction
Human corneal epithelial cell
Tribology

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

Online availability

10.1007/s10529-007-9623-z

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title = "A novel method for low load friction testing on living cells",

abstract = "A low load tribology technique for studying the effects of friction on living cells was developed. Results show a direct relationship between the coefficient of friction (COF) and the extent of cell damage. The COF, μ, for a glass pin on an intact layer of human corneal epithelial cells is determined to be on the order of μ = 0.05 ± 0.02 (n = 16). The correlations between applied normal load and extent of cell damage, as well as between number of reciprocation cycles and cell damage, are reported. It is also found that cell damage can occur when a loading force as low as 0.5 mN is applied, although the cells appear to be intact.",

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AU - Cobb, Jessica A.

AU - Dunn, Alison C.

AU - Kwon, Jiwoon

AU - Sarntinoranont, Malisa

AU - Sawyer, W. Gregory

AU - Tran-Son-Tay, Roger

PY - 2008/5

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AB - A low load tribology technique for studying the effects of friction on living cells was developed. Results show a direct relationship between the coefficient of friction (COF) and the extent of cell damage. The COF, μ, for a glass pin on an intact layer of human corneal epithelial cells is determined to be on the order of μ = 0.05 ± 0.02 (n = 16). The correlations between applied normal load and extent of cell damage, as well as between number of reciprocation cycles and cell damage, are reported. It is also found that cell damage can occur when a loading force as low as 0.5 mN is applied, although the cells appear to be intact.

KW - Cell

KW - Friction

KW - Human corneal epithelial cell

KW - Tribology

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A novel method for low load friction testing on living cells

Abstract

Keywords

ASJC Scopus subject areas

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