A novel high-throughput multi-parameter flow cytometry based method for monitoring and rapid characterization of microbiome dynamics in anaerobic systems

Abhishek S. Dhoble, Sadia Bekal, William Dolatowski, Connor Yanz, Kris N. Lambert, Kaustubh D. Bhalerao

Research output: Contribution to journalArticle

Abstract

A novel multidimensional flow cytometry based method has been demonstrated to monitor and rapidly characterize the dynamics of the complex anaerobic microbiome associated with perturbations in external environmental factors. While community fingerprinting provides an estimate of the meta genomic structure, flow cytometry provides a fingerprint of the community morphology including its autofluorescence spectrum in a high-throughput manner. Using anaerobic microbial consortia perturbed with the controlled addition of various carbon sources, it is possible to quantitatively discriminate between divergent microbiome analogous to community fingerprinting techniques using automated ribosomal intergenic spacer analysis (ARISA). The utility of flow cytometry based method has also been demonstrated in a fully functional industry scale anaerobic digester to distinguish between microbiome composition caused by varying hydraulic retention time (HRT). This approach exploits the rich multidimensional information from flow cytometry for rapid characterization of the dynamics of microbial communities.

Original languageEnglish (US)
Pages (from-to)566-571
Number of pages6
JournalBioresource Technology
Volume220
DOIs
StatePublished - Nov 1 2016

Keywords

  • Anaerobic digestion
  • Autofluorescence
  • Flow cytometry
  • Labe free monitoring
  • Microbial community
  • Microbial dynamics
  • Microbiome characterization

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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