A Novel Cell Traction Force Microscopy to Study Multi-Cellular System

Xin Tang, Alireza Tofangchi, Sandeep V. Anand, M Taher A Saif

Research output: Contribution to journalArticle

Abstract

Traction forces exerted by adherent cells on their microenvironment can mediate many critical cellular functions. Accurate quantification of these forces is essential for mechanistic understanding of mechanotransduction. However, most existing methods of quantifying cellular forces are limited to single cells in isolation, whereas most physiological processes are inherently multi-cellular in nature where cell-cell and cell-microenvironment interactions determine the emergent properties of cell clusters. In the present study, a robust finite-element-method-based cell traction force microscopy technique is developed to estimate the traction forces produced by multiple isolated cells as well as cell clusters on soft substrates. The method accounts for the finite thickness of the substrate. Hence, cell cluster size can be larger than substrate thickness. The method allows computing the traction field from the substrate displacements within the cells' and clusters' boundaries. The displacement data outside these boundaries are not necessary. The utility of the method is demonstrated by computing the traction generated by multiple monkey kidney fibroblasts (MKF) and human colon cancerous (HCT-8) cells in close proximity, as well as by large clusters. It is found that cells act as individual contractile groups within clusters for generating traction. There may be multiple of such groups in the cluster, or the entire cluster may behave a single group. Individual cells do not form dipoles, but serve as a conduit of force (transmission lines) over long distances in the cluster. The cell-cell force can be either tensile or compressive depending on the cell-microenvironment interactions.

Original languageEnglish (US)
Article numbere1003631
JournalPLoS Computational Biology
Volume10
Issue number6
DOIs
StatePublished - Jun 2014

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traction (mechanics)
Cellular Systems
Atomic Force Microscopy
Traction
Microscopy
microscopy
Microscopic examination
substrate
Cell
Substrates
cells
Fibroblasts
finite element method
Electric lines
Cellular Microenvironment
Substrate
Finite element method
Cell Communication
method
Physiological Phenomena

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Modeling and Simulation
  • Ecology
  • Molecular Biology
  • Genetics
  • Cellular and Molecular Neuroscience
  • Computational Theory and Mathematics

Cite this

A Novel Cell Traction Force Microscopy to Study Multi-Cellular System. / Tang, Xin; Tofangchi, Alireza; Anand, Sandeep V.; Saif, M Taher A.

In: PLoS Computational Biology, Vol. 10, No. 6, e1003631, 06.2014.

Research output: Contribution to journalArticle

Tang, Xin ; Tofangchi, Alireza ; Anand, Sandeep V. ; Saif, M Taher A. / A Novel Cell Traction Force Microscopy to Study Multi-Cellular System. In: PLoS Computational Biology. 2014 ; Vol. 10, No. 6.
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