Abstract
The fabrication and characteristics of a novel multiunit recording electrode and modified brain slice chamber suitable for long-term recording from brain slices are described. The electrode consisted of an electrolyte-filled glass micropipette with a 20-50 μm thick wax-coated bundle of 5-μm diameter carbon fibers extending 2.5 cm from the tapered end and an AgCl-coated silver wire inserted into the open end and connected to a preamplifier. Both ends of the electrode were sealed with wax to prevent evaporation of the electrolyte. The brain slice was maintained over this extended period in an interface-type brain slice chamber modified to completely surround the slice with medium. Using this electrode, regular 24-h oscillations of spontaneous multiunit activity were recorded for 3 days from a single location in a 500 μm thick rat suprachiasmatic nucleus brain slice. Preliminary data suggest that this novel carbon fiber bundle electrode will be a favorable alternative to traditional meter electrodes for long-term recording of multiunit activity from brain slices.
Original language | English (US) |
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Pages (from-to) | 163-169 |
Number of pages | 7 |
Journal | Journal of Neuroscience Methods |
Volume | 69 |
Issue number | 2 |
DOIs | |
State | Published - Nov 1996 |
Keywords
- Brain slice
- Carbon fiber
- Circadian rhythm
- Electrode
- Hypothalamus
- Long-term recording
- Multiunit activity
- Suprachiasmatic nucleus
ASJC Scopus subject areas
- General Neuroscience