A novel and sensitive method for recognition and indirect determination of AlIII in biological fluid based on the quenching of resonance Rayleigh scattering intensities of "AlIII-EV-DNA" complexing system

Xiufen Long, Desheng Li, Na Wang, Caihua Zhang, Qing Cao, Tao Xiancong, Shuping Bi

Research output: Contribution to journalArticle

Abstract

A novel method for recognition and indirect determination of AlIII by using biological molecules has been established based on the quenching of RRS intensity. In the weak acidic medium, the reaction of ethyl violet (EV) and DNA would result in great enhancement of RRS intensity. However, the presence of AlIII would lead to the decrease of the RRS intensity owing to the competition coordination of Al with DNA. The decreased intensity of RRS is directly proportional to the concentration of AlIII in the range of (0.1-2.5) × 10-6 and (0.30-4.5) × 10-5 M, respectively. The method has high sensitivity and its detection limit (3σ) is 3.6 × 10-8 M. The characteristics of RRS spectra of the system, the optimum conditions of the reaction, and the reaction mechanism have been investigated. The method can recognize AlIII selectively owing to its strong binding to the phosphate backbone of DNA, and has been applied to the determination of AlIII concentration in synthetic biological samples with satisfactory results. Therefore, the proposed method is promising as an effective means for selective recognition and sensitive determination in situ of AlIII. Furthermore, this study would contribute to further understanding of the biological significance of Al neurotoxicity.

Original languageEnglish (US)
Pages (from-to)142-147
Number of pages6
JournalSpectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume69
Issue number1
DOIs
StatePublished - Jan 1 2008
Externally publishedYes

Fingerprint

Rayleigh scattering
resonance scattering
Quenching
DNA
deoxyribonucleic acid
quenching
Fluids
fluids
Phosphates
phosphates
Molecules
ethyl violet
augmentation
sensitivity
molecules

Keywords

  • Al
  • DNA
  • Ethyl violet (EV)
  • RRS

ASJC Scopus subject areas

  • Analytical Chemistry
  • Atomic and Molecular Physics, and Optics
  • Instrumentation
  • Spectroscopy

Cite this

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title = "A novel and sensitive method for recognition and indirect determination of AlIII in biological fluid based on the quenching of resonance Rayleigh scattering intensities of {"}AlIII-EV-DNA{"} complexing system",
abstract = "A novel method for recognition and indirect determination of AlIII by using biological molecules has been established based on the quenching of RRS intensity. In the weak acidic medium, the reaction of ethyl violet (EV) and DNA would result in great enhancement of RRS intensity. However, the presence of AlIII would lead to the decrease of the RRS intensity owing to the competition coordination of Al with DNA. The decreased intensity of RRS is directly proportional to the concentration of AlIII in the range of (0.1-2.5) × 10-6 and (0.30-4.5) × 10-5 M, respectively. The method has high sensitivity and its detection limit (3σ) is 3.6 × 10-8 M. The characteristics of RRS spectra of the system, the optimum conditions of the reaction, and the reaction mechanism have been investigated. The method can recognize AlIII selectively owing to its strong binding to the phosphate backbone of DNA, and has been applied to the determination of AlIII concentration in synthetic biological samples with satisfactory results. Therefore, the proposed method is promising as an effective means for selective recognition and sensitive determination in situ of AlIII. Furthermore, this study would contribute to further understanding of the biological significance of Al neurotoxicity.",
keywords = "Al, DNA, Ethyl violet (EV), RRS",
author = "Xiufen Long and Desheng Li and Na Wang and Caihua Zhang and Qing Cao and Tao Xiancong and Shuping Bi",
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TY - JOUR

T1 - A novel and sensitive method for recognition and indirect determination of AlIII in biological fluid based on the quenching of resonance Rayleigh scattering intensities of "AlIII-EV-DNA" complexing system

AU - Long, Xiufen

AU - Li, Desheng

AU - Wang, Na

AU - Zhang, Caihua

AU - Cao, Qing

AU - Xiancong, Tao

AU - Bi, Shuping

PY - 2008/1/1

Y1 - 2008/1/1

N2 - A novel method for recognition and indirect determination of AlIII by using biological molecules has been established based on the quenching of RRS intensity. In the weak acidic medium, the reaction of ethyl violet (EV) and DNA would result in great enhancement of RRS intensity. However, the presence of AlIII would lead to the decrease of the RRS intensity owing to the competition coordination of Al with DNA. The decreased intensity of RRS is directly proportional to the concentration of AlIII in the range of (0.1-2.5) × 10-6 and (0.30-4.5) × 10-5 M, respectively. The method has high sensitivity and its detection limit (3σ) is 3.6 × 10-8 M. The characteristics of RRS spectra of the system, the optimum conditions of the reaction, and the reaction mechanism have been investigated. The method can recognize AlIII selectively owing to its strong binding to the phosphate backbone of DNA, and has been applied to the determination of AlIII concentration in synthetic biological samples with satisfactory results. Therefore, the proposed method is promising as an effective means for selective recognition and sensitive determination in situ of AlIII. Furthermore, this study would contribute to further understanding of the biological significance of Al neurotoxicity.

AB - A novel method for recognition and indirect determination of AlIII by using biological molecules has been established based on the quenching of RRS intensity. In the weak acidic medium, the reaction of ethyl violet (EV) and DNA would result in great enhancement of RRS intensity. However, the presence of AlIII would lead to the decrease of the RRS intensity owing to the competition coordination of Al with DNA. The decreased intensity of RRS is directly proportional to the concentration of AlIII in the range of (0.1-2.5) × 10-6 and (0.30-4.5) × 10-5 M, respectively. The method has high sensitivity and its detection limit (3σ) is 3.6 × 10-8 M. The characteristics of RRS spectra of the system, the optimum conditions of the reaction, and the reaction mechanism have been investigated. The method can recognize AlIII selectively owing to its strong binding to the phosphate backbone of DNA, and has been applied to the determination of AlIII concentration in synthetic biological samples with satisfactory results. Therefore, the proposed method is promising as an effective means for selective recognition and sensitive determination in situ of AlIII. Furthermore, this study would contribute to further understanding of the biological significance of Al neurotoxicity.

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