A nonradiometric, high-throughput assay for poly(ADP-ribose) glycohydrolase (PARG): Application to inhibitor identification and evaluation

Keyphrases

• High-throughput Assay 100%
• Poly(ADP-ribose) Glycohydrolase 100%
• Inhibitor Identification 100%
• Inhibitor Evaluation 100%
• ADP-ribose 30%
• Small Molecule Inhibitors 20%
• Poly(ADP-ribose) Polymerase 20%
• Highly Sensitive 10%
• High-throughput 10%
• Fluorescent Dye 10%
• Enzymatic Activity 10%
• Hydrolysis 10%
• High-throughput Screening 10%
• Ischemia-reperfusion Injury 10%
• Toxicity Issues 10%
• Cell Permeability 10%
• IC50 Value 10%
• In Vitro Assessment 10%
• Non-radioactive 10%
• Catalyzed Reaction 10%
• Glycosidic Bond 10%
• Poly(ADP-ribose) 10%
• Cellular Models 10%
• 384-well Plate 10%
• Neuronal Cell Death 10%
• Radiolabeled Substrates 10%
• Product Quantity 10%
• Plate Assay 10%
• TLC Analysis 10%

Pharmacology, Toxicology and Pharmaceutical Science

• Poly(adenosine Diphosphate Ribose) 100%
• Glycosidase 100%
• Adenosine Diphosphate Ribose 30%
• Nicotinamide Adenine Dinucleotide Adenosine Diphosphate Ribosyltransferase 20%
• IC50 10%
• Fluorescent Dye 10%
• Reperfusion Injury 10%
• High-Throughput Screening 10%
• Adenosine Diphosphate 10%
• Glycosidic Bond 10%