A NIR Light Gated DNA Nanodevice for Spatiotemporally Controlled Imaging of MicroRNA in Cells and Animals

Jian Zhao, Hongqian Chu, Ya Zhao, Yi Lu, Lele Li

Research output: Contribution to journalArticle

Abstract

Nanodevices have potential as intelligent sensing systems for detection of microRNAs (miRNAs) in living cells. However, the resolution offered by "always active" nanodevices is often insufficient to manipulate miRNA sensing with high spatiotemporal control. In this work, using DNA nanotechnology we constructed an activatable DNA nanodevice programmed to detect miRNAs in vitro and in vivo with the high spatial and temporal precision of NIR light. Our nanodevice is functionalized on the surface of upconversion nanoparticles (UCNPs) with a rationally designed DNA beacon that displays UV light-activatable miRNA sensing activity. The UCNPs absorb deep-tissue-penetrable NIR light and emit high-energy UV light locally, which serve as transducers to operate the nanodevice in the NIR window. The nanodevice can naturally enter cells and enable remote regulation of its fluorescent imaging activity for miRNAs in living cells by NIR light illumination in a chosen place and time. Furthermore, we demonstrate that the nanodevice can be expanded to activatable imaging of intratumoral miRNAs in living mice. This work illustrates the potential of DNA nanodevices for miRNA detection with high spatiotemporal resolution, which could expand the toolbox of technologies for precise biological and medical analysis. ©

Original languageEnglish (US)
Pages (from-to)7056-7062
Number of pages7
JournalJournal of the American Chemical Society
Volume141
Issue number17
DOIs
StatePublished - May 1 2019

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MicroRNAs
Animals
DNA
Imaging techniques
Light
animal
Ultraviolet radiation
Cells
Nanoparticles
Ultraviolet Rays
Nanotechnology
nanotechnology
Transducers
transducer
Lighting
Tissue
Technology
energy
detection
nanoparticle

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

A NIR Light Gated DNA Nanodevice for Spatiotemporally Controlled Imaging of MicroRNA in Cells and Animals. / Zhao, Jian; Chu, Hongqian; Zhao, Ya; Lu, Yi; Li, Lele.

In: Journal of the American Chemical Society, Vol. 141, No. 17, 01.05.2019, p. 7056-7062.

Research output: Contribution to journalArticle

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