A New Method for Label-Free Imaging of Biomolecular Interactions

Peter Y. Li, Bo Lin, John Gerstenmaier, Brian T Cunningham

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A new method for producing label-free high-resolution images of biomolecular binding to the surface of a biosensor is demonstrated. The biosensor incorporates a guided mode resonance filter that, when illuminated with white light, is designed to reflect only a narrow band of wavelengths where the reflected wavelength is tuned by the adsorption of biological material onto the sensor surface. In this work, an instrument is used to acquire spatial maps of the resonant reflected wavelength in order to produce images of bound biomolecule patterns as applied by a microarray spotter. The biosensor imaging capability is expected to yield applications in pharmaceutical compound screening, genomics, proteomics, and molecular diagnostics, where there is a need to screen large numbers of biochemical interactions against samples using low volumes of reagents.

Original languageEnglish (US)
Title of host publicationProceedings of IEEE Sensors
Pages310-315
Number of pages6
Volume2
Edition1
StatePublished - Dec 1 2003
Externally publishedYes
EventSecond International Conference on Sensors: IEEE Sensors 2003 - Toronto, Ont., Canada
Duration: Oct 22 2003Oct 24 2003

Other

OtherSecond International Conference on Sensors: IEEE Sensors 2003
CountryCanada
CityToronto, Ont.
Period10/22/0310/24/03

Keywords

  • Imaging
  • Label-free
  • Optical biosensor
  • SPR

ASJC Scopus subject areas

  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering

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