A new method for label-free imaging of biomolecular interactions

Peter Y. Li, Bo Lin, John Gerstenmaier, Brian T. Cunningham

Research output: Contribution to journalArticle

Abstract

A new method for producing label-free high-resolution images of biomolecular binding to the surface of a biosensor is demonstrated. The biosensor incorporates a guided-mode resonance filter that, when illuminated with white light, is designed to reflect only a narrow band of wavelengths where the reflected wavelength is tuned by the adsorption of biological material onto the sensor surface. In this work, an instrument is used to acquire spatial maps of the resonant reflected wavelength in order to produce images of bound biomolecule patterns as applied by a microarray spotter. The biosensor imaging capability is expected to yield applications in pharmaceutical compound screening, genomics, proteomics, and molecular diagnostics, where there is a need to screen large numbers of biochemical interactions against samples using low volumes of reagents.

Original languageEnglish (US)
Pages (from-to)6-13
Number of pages8
JournalSensors and Actuators, B: Chemical
Volume99
Issue number1
DOIs
StatePublished - Apr 15 2004
Externally publishedYes

Keywords

  • Biomolecular interactions
  • Label-free imaging
  • Surface plasmon resonance (SPR)

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry
  • Electrical and Electronic Engineering

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