A New Era of Genome Integration - Simply Cut and Paste!

Zihe Liu, Youyun Liang, Ee Lui Ang, Huimin Zhao

Research output: Contribution to journalReview articlepeer-review


Genome integration is a powerful tool in both basic and applied biological research. However, traditional genome integration, which is typically mediated by homologous recombination, has been constrained by low efficiencies and limited host range. In recent years, the emergence of homing endonucleases and programmable nucleases has greatly enhanced integration efficiencies and allowed alternative integration mechanisms such as nonhomologous end joining and microhomology-mediated end joining, enabling integration in hosts deficient in homologous recombination. In this review, we will highlight recent advances and breakthroughs in genome integration methods made possible by programmable nucleases, and their new applications in synthetic biology and metabolic engineering.

Original languageEnglish (US)
Pages (from-to)601-609
Number of pages9
JournalACS synthetic biology
Issue number4
StatePublished - Apr 21 2017


  • genome integration
  • metabolic engineering
  • programmable nucleases
  • synthetic biology

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)


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