A New Era of Genome Integration - Simply Cut and Paste!

Zihe Liu, Youyun Liang, Ee Lui Ang, Huimin Zhao

Research output: Contribution to journalReview article

Abstract

Genome integration is a powerful tool in both basic and applied biological research. However, traditional genome integration, which is typically mediated by homologous recombination, has been constrained by low efficiencies and limited host range. In recent years, the emergence of homing endonucleases and programmable nucleases has greatly enhanced integration efficiencies and allowed alternative integration mechanisms such as nonhomologous end joining and microhomology-mediated end joining, enabling integration in hosts deficient in homologous recombination. In this review, we will highlight recent advances and breakthroughs in genome integration methods made possible by programmable nucleases, and their new applications in synthetic biology and metabolic engineering.

Original languageEnglish (US)
Pages (from-to)601-609
Number of pages9
JournalACS synthetic biology
Volume6
Issue number4
DOIs
StatePublished - Apr 21 2017

Fingerprint

Ointments
Genes
Homologous Recombination
Genome
Synthetic Biology
Metabolic Engineering
Endonucleases
Host Specificity
Joining
Metabolic engineering
Research

Keywords

  • genome integration
  • metabolic engineering
  • programmable nucleases
  • synthetic biology

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

Cite this

A New Era of Genome Integration - Simply Cut and Paste! / Liu, Zihe; Liang, Youyun; Ang, Ee Lui; Zhao, Huimin.

In: ACS synthetic biology, Vol. 6, No. 4, 21.04.2017, p. 601-609.

Research output: Contribution to journalReview article

Liu, Zihe ; Liang, Youyun ; Ang, Ee Lui ; Zhao, Huimin. / A New Era of Genome Integration - Simply Cut and Paste!. In: ACS synthetic biology. 2017 ; Vol. 6, No. 4. pp. 601-609.
@article{9130b0a7ae7c4277a896f4668d85251e,
title = "A New Era of Genome Integration - Simply Cut and Paste!",
abstract = "Genome integration is a powerful tool in both basic and applied biological research. However, traditional genome integration, which is typically mediated by homologous recombination, has been constrained by low efficiencies and limited host range. In recent years, the emergence of homing endonucleases and programmable nucleases has greatly enhanced integration efficiencies and allowed alternative integration mechanisms such as nonhomologous end joining and microhomology-mediated end joining, enabling integration in hosts deficient in homologous recombination. In this review, we will highlight recent advances and breakthroughs in genome integration methods made possible by programmable nucleases, and their new applications in synthetic biology and metabolic engineering.",
keywords = "genome integration, metabolic engineering, programmable nucleases, synthetic biology",
author = "Zihe Liu and Youyun Liang and Ang, {Ee Lui} and Huimin Zhao",
year = "2017",
month = "4",
day = "21",
doi = "10.1021/acssynbio.6b00331",
language = "English (US)",
volume = "6",
pages = "601--609",
journal = "ACS Synthetic Biology",
issn = "2161-5063",
publisher = "American Chemical Society",
number = "4",

}

TY - JOUR

T1 - A New Era of Genome Integration - Simply Cut and Paste!

AU - Liu, Zihe

AU - Liang, Youyun

AU - Ang, Ee Lui

AU - Zhao, Huimin

PY - 2017/4/21

Y1 - 2017/4/21

N2 - Genome integration is a powerful tool in both basic and applied biological research. However, traditional genome integration, which is typically mediated by homologous recombination, has been constrained by low efficiencies and limited host range. In recent years, the emergence of homing endonucleases and programmable nucleases has greatly enhanced integration efficiencies and allowed alternative integration mechanisms such as nonhomologous end joining and microhomology-mediated end joining, enabling integration in hosts deficient in homologous recombination. In this review, we will highlight recent advances and breakthroughs in genome integration methods made possible by programmable nucleases, and their new applications in synthetic biology and metabolic engineering.

AB - Genome integration is a powerful tool in both basic and applied biological research. However, traditional genome integration, which is typically mediated by homologous recombination, has been constrained by low efficiencies and limited host range. In recent years, the emergence of homing endonucleases and programmable nucleases has greatly enhanced integration efficiencies and allowed alternative integration mechanisms such as nonhomologous end joining and microhomology-mediated end joining, enabling integration in hosts deficient in homologous recombination. In this review, we will highlight recent advances and breakthroughs in genome integration methods made possible by programmable nucleases, and their new applications in synthetic biology and metabolic engineering.

KW - genome integration

KW - metabolic engineering

KW - programmable nucleases

KW - synthetic biology

UR - http://www.scopus.com/inward/record.url?scp=85018466549&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85018466549&partnerID=8YFLogxK

U2 - 10.1021/acssynbio.6b00331

DO - 10.1021/acssynbio.6b00331

M3 - Review article

C2 - 28073246

AN - SCOPUS:85018466549

VL - 6

SP - 601

EP - 609

JO - ACS Synthetic Biology

JF - ACS Synthetic Biology

SN - 2161-5063

IS - 4

ER -