A new assessment of the cytotoxicity and genotoxicity of drinking water disinfection by-products

Yahya Kargalioglu, Brian J. McMillan, Roger A. Minear, Michael J. Plewa

Research output: Contribution to journalArticle

Abstract

The disinfection of drinking water generates cytotoxic and mutagenic compounds. The cytotoxic and mutagenic properties of known disinfection by-products (DBPs) were quantitatively compared. Using Salmonella typhimurium strain TA100 a rapid, semi-automated, microplate cytotoxicity assay was developed. The assay can accommodate a concentration range of six log orders of magnitude with 6 replicates per concentration and requires approximately 5 h. Data were automatically transferred from a microplate reader to a computer spreadsheet. The DBP concentration that induced 50% repression of growth in the cytotoxicity assay was used as the highest concentration for the S. typhimurium mutagenicity assay. Selected DBPs were assayed in S. typhimurium strains TA98, TA100 and RSJ100 under suspension test conditions. The mutagenic potency of the DBPs were calculated and compared with the cytotoxicity data.

Original languageEnglish (US)
Pages (from-to)16-27
Number of pages12
JournalACS Symposium Series
Volume761
DOIs
StatePublished - Jan 1 2000

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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