A neutron reflectivity study of polymer-modified phospholipid monolayers at the solid-solution interface: Polyethylene glycol-lipids on silane- modified substrates

T. L. Kuhl, J. Majewski, J. Y. Wong, S. Steinberg, D. E. Leckband, J. N. Israelachvili, G. S. Smith

Research output: Contribution to journalArticle

Abstract

The structure of polymer-decorated phospholipid monotayers at the solid- solution interface was investigated using neutron reflectometry. The monolayers were composed of distearoylphosphatidylethanolamine (DSPE) matrixed with varying amounts of DSPE-PEG (DSPE with polyethylene glycol covalently grafted to its headgroup). Mixed lipid monolayers were Langmuir- Blodgett deposited onto hydrophobic quartz or silicon substrates, previously hydrophobized by chemically grafting a robust monolayer of octadecyltrichlorosilane (OTS). We show that this method results in homogeneous and continuous phospholipid monolayers on the silanated substrates and determine that the grafted PEG chains extend away from the monolayers into the solvent phase as a function of their density, as expected from scaling theories. In addition, ligands were coupled to the end of the PEG chains and selective binding was demonstrated using fluorescence microscopy. Our results demonstrate that these constructs are ideal for further characterization and studies with well-defined monomolecular films.

Original languageEnglish (US)
Pages (from-to)2352-2362
Number of pages11
JournalBiophysical journal
Volume75
Issue number5
DOIs
StatePublished - Nov 1998

Fingerprint

Silanes
Neutrons
Phospholipids
Polymers
Lipids
Quartz
Silicon
Fluorescence Microscopy
Ligands
octadecyltrichlorosilane
polyethylene glycol-distearoylphosphatidylethanolamine

ASJC Scopus subject areas

  • Biophysics

Cite this

A neutron reflectivity study of polymer-modified phospholipid monolayers at the solid-solution interface : Polyethylene glycol-lipids on silane- modified substrates. / Kuhl, T. L.; Majewski, J.; Wong, J. Y.; Steinberg, S.; Leckband, D. E.; Israelachvili, J. N.; Smith, G. S.

In: Biophysical journal, Vol. 75, No. 5, 11.1998, p. 2352-2362.

Research output: Contribution to journalArticle

Kuhl, T. L. ; Majewski, J. ; Wong, J. Y. ; Steinberg, S. ; Leckband, D. E. ; Israelachvili, J. N. ; Smith, G. S. / A neutron reflectivity study of polymer-modified phospholipid monolayers at the solid-solution interface : Polyethylene glycol-lipids on silane- modified substrates. In: Biophysical journal. 1998 ; Vol. 75, No. 5. pp. 2352-2362.
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