A neonatal piglet model for investigating brain and cognitive development in small for gestational age human infants

Emily C. Radlowski, Matthew S. Conrad, Stephane Lezmi, Ryan N. Dilger, Brad Sutton, Ryan Larsen, Rodney W. Johnson

Research output: Contribution to journalArticle

Abstract

The piglet was investigated as a potential model for studying brain and cognitive deficits associated with being born small for gestational age (SGA). Naturally farrowed SGA (0.7-1.0 kg BW) and average for gestational age (AGA, 1.3-1.6 kg BW) piglets were obtained on postnatal day (PD) 2, placed in individual cages, and provided a nutritionally adequate milk replacer diet (285 ml/kg/d). Beginning at PD14, performance in a spatial T-maze task was assessed. At PD28, piglets were anesthetized for magnetic resonance (MR) imaging to assess brain structure (voxel-based morphometry), connectivity (diffusion-tensor imaging) and metabolites in the hippocampus and corpus callosum (proton MR spectroscopy). Piglets born SGA showed compensatory growth such that BW of SGA and AGA piglets was similar (P>0.05), by PD15. Birth weight affected maze performance, with SGA piglets taking longer to reach criterion than AGA piglets (p<0.01). Total brain volume of SGA and AGA piglets was similar (P<0.05), but overall, SGA piglets had less gray matter than AGA piglets (p<0.01) and tended to have a smaller internal capsule (p = 0.07). Group comparisons between SGA and AGA piglets defined 9 areas (> 20 clusters) where SGA piglets had less white matter (p<0.01); 2 areas where SGA piglets had more white matter (p<0.01); and 3 areas where SGA piglets had more gray matter (p<0.01). The impact of being born SGA on white matter was supported by a lower (p<0.04) fractional anisotropy value for SGA piglets, suggesting reduced white matter development and connectivity. None of the metabolites measured were different between groups. Collectively, the results show that SGA piglets have spatial learning deficits and abnormal development of white matter. As learning deficits and abnormalities in white matter are common in SGA human infants, the piglet is a tractable translational model that can be used to investigate SGA-associated cognitive deficits and potential interventions. Copyright:

Original languageEnglish (US)
Article numbere91951
JournalPloS one
Volume9
Issue number3
DOIs
StatePublished - Mar 17 2014

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small for gestational age
Small for Gestational Age Infant
cognitive development
Metabolites
Gestational Age
piglets
Brain
Diffusion tensor imaging
Magnetic resonance spectroscopy
brain
Magnetic resonance
Nutrition
Anisotropy
Nuclear magnetic resonance
Imaging techniques
learning
metabolites
Diffusion Tensor Imaging
milk replacer
compensatory growth

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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A neonatal piglet model for investigating brain and cognitive development in small for gestational age human infants. / Radlowski, Emily C.; Conrad, Matthew S.; Lezmi, Stephane; Dilger, Ryan N.; Sutton, Brad; Larsen, Ryan; Johnson, Rodney W.

In: PloS one, Vol. 9, No. 3, e91951, 17.03.2014.

Research output: Contribution to journalArticle

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