TY - JOUR
T1 - A murine model of pseudorabies virus latency
AU - Osorio, F. A.
AU - Rock, D. L.
N1 - Funding Information:
This project was partly supported by United States Department of Agriculture (USDA) Special Research Grant (Animal Health) No . 89-01939, and by a grant from the National Pork Producers Council . This manuscript has been assigned Journal Series No . 9694, Agricultural Research Division, University of Nebraska .
PY - 1992/1
Y1 - 1992/1
N2 - The mouse is a useful laboratory animal for studying various aspects of pseudorabies virus (PRV) virulence. Mice are highly susceptible hosts for PRV infection and are unable to survive acute viral infection. Because of this, mouse models have not been useful for studying PRV latent infections. Here, we report an efficient strategy for establishing latent PRV infections in laboratory mice. Passive transfer of high titered neutralizing antibodies to mice prior to inoculation with highly lethal doses of PRV (Bartha) resulted in survival rates of at least 60% with establishment of latent infections in survivors. Latent PRV infection in mice was demonstrated by: (1) recovery of infectious PRV-Bartha from explants of trigeminal ganglion (TG), and (2) detection of PRV nucleic acids in latently infected TGs by in situ hybridization and polymerase chain reaction (PCR), between 2-8 months post-infection. This PRV latency model indicates that attenuated PRV strains, those currently used extensively in vaccination programs worldwide, can establish a reactivatable latent infection in an experimental host. The mouse model may be particularly useful for examining the molecular bases of PRV latency and reactivation.
AB - The mouse is a useful laboratory animal for studying various aspects of pseudorabies virus (PRV) virulence. Mice are highly susceptible hosts for PRV infection and are unable to survive acute viral infection. Because of this, mouse models have not been useful for studying PRV latent infections. Here, we report an efficient strategy for establishing latent PRV infections in laboratory mice. Passive transfer of high titered neutralizing antibodies to mice prior to inoculation with highly lethal doses of PRV (Bartha) resulted in survival rates of at least 60% with establishment of latent infections in survivors. Latent PRV infection in mice was demonstrated by: (1) recovery of infectious PRV-Bartha from explants of trigeminal ganglion (TG), and (2) detection of PRV nucleic acids in latently infected TGs by in situ hybridization and polymerase chain reaction (PCR), between 2-8 months post-infection. This PRV latency model indicates that attenuated PRV strains, those currently used extensively in vaccination programs worldwide, can establish a reactivatable latent infection in an experimental host. The mouse model may be particularly useful for examining the molecular bases of PRV latency and reactivation.
KW - Aujeszky's disease virus
KW - herpes virus latency
KW - in situ hybridization
KW - polymerase chain reaction
KW - pseudorabies virus
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U2 - 10.1016/0882-4010(92)90064-U
DO - 10.1016/0882-4010(92)90064-U
M3 - Article
C2 - 1313942
AN - SCOPUS:0026479781
SN - 0882-4010
VL - 12
SP - 39
EP - 46
JO - Microbial Pathogenesis
JF - Microbial Pathogenesis
IS - 1
ER -