A multiplexed microfluidic platform utilizing optimized normally closed valves for investigating microbial gene expression

Ritika Mohan, Arnab Mukherjee, Amit V. Desai, Jaebum Lee, Charles M. Schroeder, Paul J.A. Kenis

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports the development of a microfluidic platform that utilizes arrays of normally closed (NC) microvalves, and its application in quantitative biological measurements, including microbial gene expression and stimulus-driven responses in living cells. The dimensions of the NC valves used in this platform were first optimized to actuate reliably at low negative pressures (-1 to -3 psig). The platform was then employed to investigate (i) inducible gene expression in Escherichia coli and (ii) the effect of an antibiotic (ampicillin) on cell growth and death.

Original languageEnglish (US)
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1014-1016
Number of pages3
StatePublished - 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: Oct 2 2011Oct 6 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume2

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Country/TerritoryUnited States
CitySeattle, WA
Period10/2/1110/6/11

Keywords

  • Antibiotics
  • Green fluorescent protein (GFP)
  • Normally closed microvalve
  • Soft lithography

ASJC Scopus subject areas

  • Control and Systems Engineering

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