Pseudomonas aeruginosa (P. aeruginosa) is a multidrug-resistant human pathogen involved in numerous infections. Understanding the response of P. aeruginosa to various treatments is critical to developing new ways for the antimicrobial susceptibly test and more effective treatment methods. Conventional antimicrobial susceptibility tests lack molecular information at the single bacterium level. In this study, we used label-free multimodal nonlinear optical microscopy to identify an autofluorescence signal from pyoverdine, a siderophore of the bacteria, for quantification of P. aeruginosa responses to antibiotics and blue light treatment. We also discovered that the bleaching of the pyoverdine autofluorescence signals is correlated with the inactivation of P. aeruginosa and is perhaps one of the mechanisms involved in the blue light inactivation of P. aeruginosa.

Original languageEnglish (US)
Article numbere202300384
JournalJournal of Biophotonics
Issue number3
StatePublished - Mar 2024


  • Pseudomonas aeruginosa
  • blue light
  • coherent anti-Stokes Raman scattering
  • nonlinear optical imaging
  • two-photon excitation fluorescence

ASJC Scopus subject areas

  • General Chemistry
  • General Engineering
  • General Biochemistry, Genetics and Molecular Biology
  • General Materials Science
  • General Physics and Astronomy


Dive into the research topics of 'A multimodal nonlinear optical microscopy study of the responses of Pseudomonas aeruginosa to blue light and antibiotic treatment'. Together they form a unique fingerprint.

Cite this