TY - JOUR
T1 - A multimodal nonlinear optical microscopy study of the responses of Pseudomonas aeruginosa to blue light and antibiotic treatment
AU - Zhang, Chi
AU - Zaki, Farzana R.
AU - Won, Jungeun
AU - Boppart, Stephen A.
N1 - The authors want to thank GlaxoSmithKline for the sponsored research and equipment support of the GSK Center for Optical Molecular Imaging at the University of Illinois Urbana-Champaign. This research was also financially supported in part by grants from the National Institutes of Health (R01CA241618, R01EB023232, P41EB031772, R01CA213149, and R01EB028615) and the National Science Foundation (CBET 18-41539). Additional information can be found at http://biophotonics.illinois.edu.
The authors want to thank GlaxoSmithKline for the sponsored research and equipment support of the GSK Center for Optical Molecular Imaging at the University of Illinois Urbana\u2010Champaign. This research was also financially supported in part by grants from the National Institutes of Health (R01CA241618, R01EB023232, P41EB031772, R01CA213149, and R01EB028615) and the National Science Foundation (CBET 18\u201041539). Additional information can be found at http://biophotonics.illinois.edu .
PY - 2024/3
Y1 - 2024/3
N2 - Pseudomonas aeruginosa (P. aeruginosa) is a multidrug-resistant human pathogen involved in numerous infections. Understanding the response of P. aeruginosa to various treatments is critical to developing new ways for the antimicrobial susceptibly test and more effective treatment methods. Conventional antimicrobial susceptibility tests lack molecular information at the single bacterium level. In this study, we used label-free multimodal nonlinear optical microscopy to identify an autofluorescence signal from pyoverdine, a siderophore of the bacteria, for quantification of P. aeruginosa responses to antibiotics and blue light treatment. We also discovered that the bleaching of the pyoverdine autofluorescence signals is correlated with the inactivation of P. aeruginosa and is perhaps one of the mechanisms involved in the blue light inactivation of P. aeruginosa.
AB - Pseudomonas aeruginosa (P. aeruginosa) is a multidrug-resistant human pathogen involved in numerous infections. Understanding the response of P. aeruginosa to various treatments is critical to developing new ways for the antimicrobial susceptibly test and more effective treatment methods. Conventional antimicrobial susceptibility tests lack molecular information at the single bacterium level. In this study, we used label-free multimodal nonlinear optical microscopy to identify an autofluorescence signal from pyoverdine, a siderophore of the bacteria, for quantification of P. aeruginosa responses to antibiotics and blue light treatment. We also discovered that the bleaching of the pyoverdine autofluorescence signals is correlated with the inactivation of P. aeruginosa and is perhaps one of the mechanisms involved in the blue light inactivation of P. aeruginosa.
KW - Pseudomonas aeruginosa
KW - blue light
KW - coherent anti-Stokes Raman scattering
KW - nonlinear optical imaging
KW - two-photon excitation fluorescence
UR - http://www.scopus.com/inward/record.url?scp=85179730180&partnerID=8YFLogxK
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U2 - 10.1002/jbio.202300384
DO - 10.1002/jbio.202300384
M3 - Article
C2 - 38010357
AN - SCOPUS:85179730180
SN - 1864-063X
VL - 17
JO - Journal of Biophotonics
JF - Journal of Biophotonics
IS - 3
M1 - e202300384
ER -