A modified method for the production of 36ClL3- for use in plant nitrate transport studies

Jose Ruiz-Cristin; Amy J. Chodera; Donald P. Briskin

doi:10.1016/0003-2697(89)90732-X

A modified method for the production of ³⁶ClL₃^- for use in plant nitrate transport studies

Jose Ruiz-Cristin
, Amy J. Chodera
, Donald P. Briskin

Crop Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

A modified method for the production and purification of ³⁶ClO₃^- in high yield is described. This procedure, involving the electrolytic oxidation of ³⁶Cl^- in a cell with simple electrode design and purification of the electrolysis products (³⁶Cl^-, ³⁶ClO₃^-, and ³⁶ClO₄^-) by aqueous column chromatography, allows for the recovery of about 80% of the initial radiolabel as ³⁶ClO₃^-. The method is rapid and suitable for the production of this radiolabeled anion for use as a tracer analog for nitrate in plant membrane transport experiments.

Original language	English (US)
Pages (from-to)	146-150
Number of pages	5
Journal	Analytical Biochemistry
Volume	182
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(89)90732-X
State	Published - Oct 1989

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(89)90732-X

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Cite this

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title = "A modified method for the production of 36ClL3- for use in plant nitrate transport studies",

abstract = "A modified method for the production and purification of 36ClO3- in high yield is described. This procedure, involving the electrolytic oxidation of 36Cl- in a cell with simple electrode design and purification of the electrolysis products (36Cl-, 36ClO3-, and 36ClO4-) by aqueous column chromatography, allows for the recovery of about 80\% of the initial radiolabel as 36ClO3-. The method is rapid and suitable for the production of this radiolabeled anion for use as a tracer analog for nitrate in plant membrane transport experiments.",

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