Abstract
A modified method for the production and purification of 36ClO3- in high yield is described. This procedure, involving the electrolytic oxidation of 36Cl- in a cell with simple electrode design and purification of the electrolysis products (36Cl-, 36ClO3-, and 36ClO4-) by aqueous column chromatography, allows for the recovery of about 80% of the initial radiolabel as 36ClO3-. The method is rapid and suitable for the production of this radiolabeled anion for use as a tracer analog for nitrate in plant membrane transport experiments.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 146-150 |
| Number of pages | 5 |
| Journal | Analytical Biochemistry |
| Volume | 182 |
| Issue number | 1 |
| DOIs | |
| State | Published - Oct 1989 |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
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Ruiz-Cristin, J., Chodera, A. J., & Briskin, D. P. (1989). A modified method for the production of 36ClL3- for use in plant nitrate transport studies. Analytical Biochemistry, 182(1), 146-150. https://doi.org/10.1016/0003-2697(89)90732-X