Abstract
A modified method for the production and purification of 36ClO3- in high yield is described. This procedure, involving the electrolytic oxidation of 36Cl- in a cell with simple electrode design and purification of the electrolysis products (36Cl-, 36ClO3-, and 36ClO4-) by aqueous column chromatography, allows for the recovery of about 80% of the initial radiolabel as 36ClO3-. The method is rapid and suitable for the production of this radiolabeled anion for use as a tracer analog for nitrate in plant membrane transport experiments.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 146-150 |
| Number of pages | 5 |
| Journal | Analytical Biochemistry |
| Volume | 182 |
| Issue number | 1 |
| DOIs | |
| State | Published - Oct 1989 |
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ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
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A modified method for the production of 36ClL3- for use in plant nitrate transport studies. / Ruiz-Cristin, Jose; Chodera, Amy J.; Briskin, Donald P.
In: Analytical Biochemistry, Vol. 182, No. 1, 10.1989, p. 146-150.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A modified method for the production of 36ClL3- for use in plant nitrate transport studies
AU - Ruiz-Cristin, Jose
AU - Chodera, Amy J.
AU - Briskin, Donald P.
PY - 1989/10
Y1 - 1989/10
N2 - A modified method for the production and purification of 36ClO3- in high yield is described. This procedure, involving the electrolytic oxidation of 36Cl- in a cell with simple electrode design and purification of the electrolysis products (36Cl-, 36ClO3-, and 36ClO4-) by aqueous column chromatography, allows for the recovery of about 80% of the initial radiolabel as 36ClO3-. The method is rapid and suitable for the production of this radiolabeled anion for use as a tracer analog for nitrate in plant membrane transport experiments.
AB - A modified method for the production and purification of 36ClO3- in high yield is described. This procedure, involving the electrolytic oxidation of 36Cl- in a cell with simple electrode design and purification of the electrolysis products (36Cl-, 36ClO3-, and 36ClO4-) by aqueous column chromatography, allows for the recovery of about 80% of the initial radiolabel as 36ClO3-. The method is rapid and suitable for the production of this radiolabeled anion for use as a tracer analog for nitrate in plant membrane transport experiments.
UR - http://www.scopus.com/inward/record.url?scp=0024746615&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024746615&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(89)90732-X
DO - 10.1016/0003-2697(89)90732-X
M3 - Article
C2 - 2604039
AN - SCOPUS:0024746615
VL - 182
SP - 146
EP - 150
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 1
ER -