A modified method for the production of 36ClL3- for use in plant nitrate transport studies

Jose Ruiz-Cristin; Amy J. Chodera; Donald P. Briskin

doi:10.1016/0003-2697(89)90732-X

A modified method for the production of ³⁶ClL₃^- for use in plant nitrate transport studies

Jose Ruiz-Cristin, Amy J. Chodera, Donald P. Briskin

Crop Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

A modified method for the production and purification of ³⁶ClO₃^- in high yield is described. This procedure, involving the electrolytic oxidation of ³⁶Cl^- in a cell with simple electrode design and purification of the electrolysis products (³⁶Cl^-, ³⁶ClO₃^-, and ³⁶ClO₄^-) by aqueous column chromatography, allows for the recovery of about 80% of the initial radiolabel as ³⁶ClO₃^-. The method is rapid and suitable for the production of this radiolabeled anion for use as a tracer analog for nitrate in plant membrane transport experiments.

Original language	English (US)
Pages (from-to)	146-150
Number of pages	5
Journal	Analytical Biochemistry
Volume	182
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(89)90732-X
State	Published - Oct 1989

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Online availability

10.1016/0003-2697(89)90732-X

Library availability

Discover UIUC Full Text

Cite this

@article{7096925e7d854d789eca5672fa9fcbe1,

title = "A modified method for the production of 36ClL3- for use in plant nitrate transport studies",

abstract = "A modified method for the production and purification of 36ClO3- in high yield is described. This procedure, involving the electrolytic oxidation of 36Cl- in a cell with simple electrode design and purification of the electrolysis products (36Cl-, 36ClO3-, and 36ClO4-) by aqueous column chromatography, allows for the recovery of about 80% of the initial radiolabel as 36ClO3-. The method is rapid and suitable for the production of this radiolabeled anion for use as a tracer analog for nitrate in plant membrane transport experiments.",

author = "Jose Ruiz-Cristin and Chodera, {Amy J.} and Briskin, {Donald P.}",

year = "1989",

month = oct,

doi = "10.1016/0003-2697(89)90732-X",

language = "English (US)",

volume = "182",

pages = "146--150",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - A modified method for the production of 36ClL3- for use in plant nitrate transport studies

AU - Ruiz-Cristin, Jose

AU - Chodera, Amy J.

AU - Briskin, Donald P.

PY - 1989/10

Y1 - 1989/10

N2 - A modified method for the production and purification of 36ClO3- in high yield is described. This procedure, involving the electrolytic oxidation of 36Cl- in a cell with simple electrode design and purification of the electrolysis products (36Cl-, 36ClO3-, and 36ClO4-) by aqueous column chromatography, allows for the recovery of about 80% of the initial radiolabel as 36ClO3-. The method is rapid and suitable for the production of this radiolabeled anion for use as a tracer analog for nitrate in plant membrane transport experiments.

AB - A modified method for the production and purification of 36ClO3- in high yield is described. This procedure, involving the electrolytic oxidation of 36Cl- in a cell with simple electrode design and purification of the electrolysis products (36Cl-, 36ClO3-, and 36ClO4-) by aqueous column chromatography, allows for the recovery of about 80% of the initial radiolabel as 36ClO3-. The method is rapid and suitable for the production of this radiolabeled anion for use as a tracer analog for nitrate in plant membrane transport experiments.

UR - http://www.scopus.com/inward/record.url?scp=0024746615&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024746615&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(89)90732-X

DO - 10.1016/0003-2697(89)90732-X

M3 - Article

C2 - 2604039

AN - SCOPUS:0024746615

SN - 0003-2697

VL - 182

SP - 146

EP - 150

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -