A modified method for the production of 36ClL3- for use in plant nitrate transport studies

Jose Ruiz-Cristin, Amy J. Chodera, Donald P Briskin

Research output: Contribution to journalArticle

Abstract

A modified method for the production and purification of 36ClO3- in high yield is described. This procedure, involving the electrolytic oxidation of 36Cl- in a cell with simple electrode design and purification of the electrolysis products (36Cl-, 36ClO3-, and 36ClO4-) by aqueous column chromatography, allows for the recovery of about 80% of the initial radiolabel as 36ClO3-. The method is rapid and suitable for the production of this radiolabeled anion for use as a tracer analog for nitrate in plant membrane transport experiments.

Original languageEnglish (US)
Pages (from-to)146-150
Number of pages5
JournalAnalytical Biochemistry
Volume182
Issue number1
DOIs
StatePublished - Oct 1989

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Nitrates
Purification
Column chromatography
Electrolysis
Anions
Membranes
Recovery
Oxidation
Electrodes
Chromatography
Experiments

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

A modified method for the production of 36ClL3- for use in plant nitrate transport studies. / Ruiz-Cristin, Jose; Chodera, Amy J.; Briskin, Donald P.

In: Analytical Biochemistry, Vol. 182, No. 1, 10.1989, p. 146-150.

Research output: Contribution to journalArticle

Ruiz-Cristin, Jose ; Chodera, Amy J. ; Briskin, Donald P. / A modified method for the production of 36ClL3- for use in plant nitrate transport studies. In: Analytical Biochemistry. 1989 ; Vol. 182, No. 1. pp. 146-150.
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