A middle-affinity estrogen-specific binding protein in livers of vitellogenic and nonvitellogenic Xenopus laevis

Marshall A. Hayward, David J Shapiro

Research output: Contribution to journalArticle

Abstract

Administration of estradiol-17β to male Xenopus laevis evokes massive liver synthesis of the egg yolk precursor protein, vitellogenin, and its cognate mRNA. Since previous experiments had implicated only a nuclear estrogen receptor in vitellogenesis, we examined Xenopus liver cytosol for other estrogen-binding proteins. Cytoplasmic extracts from unstimulated Xenopus liver contain high levels (approximately 500,000 sites/cell) of an estrogen-specific binding protein. This protein exhibits a Kd of approximately 4 × 10-8M for estradiol-17β, binds estrogenic steroids only, and has a sedimentation coefficient in the range 2-3 S. It is not a classical estrogen receptor, as it does not translocate into the nucleus following estrogen administration. We discuss possible functions of this protein, which include a role in the ontogeny of the vitellogenic response, and in the cytoplasmic transport and storage of estrogen.

Original languageEnglish (US)
Pages (from-to)333-340
Number of pages8
JournalDevelopmental Biology
Volume88
Issue number2
DOIs
StatePublished - Dec 1981

Fingerprint

Xenopus laevis
Carrier Proteins
Estrogens
Liver
Xenopus
Estrogen Receptors
Estradiol
Vitellogenesis
Vitellogenins
Egg Proteins
Cytosol
Proteins
Steroids
Messenger RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

A middle-affinity estrogen-specific binding protein in livers of vitellogenic and nonvitellogenic Xenopus laevis. / Hayward, Marshall A.; Shapiro, David J.

In: Developmental Biology, Vol. 88, No. 2, 12.1981, p. 333-340.

Research output: Contribution to journalArticle

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