A microtiter plate assay for inorganic phosphate

Margarethe Hoenig; R. John Lee; Duncan C. Ferguson

doi:10.1016/0165-022X(89)90031-6

A microtiter plate assay for inorganic phosphate

Margarethe Hoenig, R. John Lee, Duncan C. Ferguson

Research output: Contribution to journal › Article › peer-review

Abstract

A microtiter assay for the detection of picomolar quantities of inorganic phosphate has been described. The assay, linear between 50 and 1000 pmol of inorganic phosphate is simple and rapid, with results obtainable in sevearl minutes. Results from 5′-nucleotidase and (Ca²⁺ + Mg²⁺)ATPase assays using this method were compared with conventional phosphate assays and showed a high degree of correlation. The high sensitivity of this assay and the small sample size needed allows its widespread use in biochemical studies involving the generation of inorganic phosphate.

Original language	English (US)
Pages (from-to)	249-251
Number of pages	3
Journal	Journal of Biochemical and Biophysical Methods
Volume	19
Issue number	2-3
DOIs	https://doi.org/10.1016/0165-022X(89)90031-6
State	Published - 1989
Externally published	Yes

Keywords

Enzyme mechanism
Inorganic analysis
Phosphatase
Phosphate assay
Phosphate compounds

ASJC Scopus subject areas

Biophysics
Biochemistry

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10.1016/0165-022X(89)90031-6

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@article{dae393128f4d4f06b819fbe6a92f580d,

title = "A microtiter plate assay for inorganic phosphate",

abstract = "A microtiter assay for the detection of picomolar quantities of inorganic phosphate has been described. The assay, linear between 50 and 1000 pmol of inorganic phosphate is simple and rapid, with results obtainable in sevearl minutes. Results from 5′-nucleotidase and (Ca2+ + Mg2+)ATPase assays using this method were compared with conventional phosphate assays and showed a high degree of correlation. The high sensitivity of this assay and the small sample size needed allows its widespread use in biochemical studies involving the generation of inorganic phosphate.",

keywords = "Enzyme mechanism, Inorganic analysis, Phosphatase, Phosphate assay, Phosphate compounds",

author = "Margarethe Hoenig and Lee, {R. John} and Ferguson, {Duncan C.}",

note = "Funding Information: The microtiter plate assay was a modification of the method described by Penney \[1\]. Reactions were carried out on Microtest III assay plates (96 U-bottom wells, Becton Dickinson and Company, Oxnard, CA). Five to twenty/~1 of sample were placed into the well and distilled water was added to give a final volume of 70 ~1. Blanks contained 70 /~1 of distilled water. Sodium molybdate (2.6 g/100 ml) was Correspondence address: M. Hoenig, Department of Physiology and Pharmacology, College of Veterinary Medicine, The University of Georgia, Athens, GA 30602, U.S.A. This study was supported in part by PHS Grant R23DK39185 (MH) and R23DK38814 (DCF).",

year = "1989",

doi = "10.1016/0165-022X(89)90031-6",

language = "English (US)",

volume = "19",

pages = "249--251",

journal = "Journal of Proteomics",

issn = "1874-3919",

publisher = "Elsevier",

number = "2-3",

}

TY - JOUR

T1 - A microtiter plate assay for inorganic phosphate

AU - Hoenig, Margarethe

AU - Lee, R. John

AU - Ferguson, Duncan C.

N1 - Funding Information: The microtiter plate assay was a modification of the method described by Penney \[1\]. Reactions were carried out on Microtest III assay plates (96 U-bottom wells, Becton Dickinson and Company, Oxnard, CA). Five to twenty/~1 of sample were placed into the well and distilled water was added to give a final volume of 70 ~1. Blanks contained 70 /~1 of distilled water. Sodium molybdate (2.6 g/100 ml) was Correspondence address: M. Hoenig, Department of Physiology and Pharmacology, College of Veterinary Medicine, The University of Georgia, Athens, GA 30602, U.S.A. This study was supported in part by PHS Grant R23DK39185 (MH) and R23DK38814 (DCF).

PY - 1989

Y1 - 1989

N2 - A microtiter assay for the detection of picomolar quantities of inorganic phosphate has been described. The assay, linear between 50 and 1000 pmol of inorganic phosphate is simple and rapid, with results obtainable in sevearl minutes. Results from 5′-nucleotidase and (Ca2+ + Mg2+)ATPase assays using this method were compared with conventional phosphate assays and showed a high degree of correlation. The high sensitivity of this assay and the small sample size needed allows its widespread use in biochemical studies involving the generation of inorganic phosphate.

AB - A microtiter assay for the detection of picomolar quantities of inorganic phosphate has been described. The assay, linear between 50 and 1000 pmol of inorganic phosphate is simple and rapid, with results obtainable in sevearl minutes. Results from 5′-nucleotidase and (Ca2+ + Mg2+)ATPase assays using this method were compared with conventional phosphate assays and showed a high degree of correlation. The high sensitivity of this assay and the small sample size needed allows its widespread use in biochemical studies involving the generation of inorganic phosphate.

KW - Enzyme mechanism

KW - Inorganic analysis

KW - Phosphatase

KW - Phosphate assay

KW - Phosphate compounds

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U2 - 10.1016/0165-022X(89)90031-6

DO - 10.1016/0165-022X(89)90031-6

M3 - Article

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AN - SCOPUS:0024464658

VL - 19

SP - 249

EP - 251

JO - Journal of Proteomics

JF - Journal of Proteomics

SN - 1874-3919

IS - 2-3

ER -

A microtiter plate assay for inorganic phosphate

Abstract

Keywords

ASJC Scopus subject areas

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