Abstract
A microtiter assay for the detection of picomolar quantities of inorganic phosphate has been described. The assay, linear between 50 and 1000 pmol of inorganic phosphate is simple and rapid, with results obtainable in sevearl minutes. Results from 5′-nucleotidase and (Ca2+ + Mg2+)ATPase assays using this method were compared with conventional phosphate assays and showed a high degree of correlation. The high sensitivity of this assay and the small sample size needed allows its widespread use in biochemical studies involving the generation of inorganic phosphate.
Original language | English (US) |
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Pages (from-to) | 249-251 |
Number of pages | 3 |
Journal | Journal of Biochemical and Biophysical Methods |
Volume | 19 |
Issue number | 2-3 |
DOIs | |
State | Published - 1989 |
Externally published | Yes |
Keywords
- Enzyme mechanism
- Inorganic analysis
- Phosphatase
- Phosphate assay
- Phosphate compounds
ASJC Scopus subject areas
- Biophysics
- Biochemistry