A microtiter plate assay for inorganic phosphate

Research output: Contribution to journalArticlepeer-review

Abstract

A microtiter assay for the detection of picomolar quantities of inorganic phosphate has been described. The assay, linear between 50 and 1000 pmol of inorganic phosphate is simple and rapid, with results obtainable in sevearl minutes. Results from 5′-nucleotidase and (Ca2+ + Mg2+)ATPase assays using this method were compared with conventional phosphate assays and showed a high degree of correlation. The high sensitivity of this assay and the small sample size needed allows its widespread use in biochemical studies involving the generation of inorganic phosphate.

Original languageEnglish (US)
Pages (from-to)249-251
Number of pages3
JournalJournal of Biochemical and Biophysical Methods
Volume19
Issue number2-3
DOIs
StatePublished - 1989
Externally publishedYes

Keywords

  • Enzyme mechanism
  • Inorganic analysis
  • Phosphatase
  • Phosphate assay
  • Phosphate compounds

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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