Abstract
The diacetyl monoxime colorimetric method of determining urea in soil extracts was modified for microplate format. A 100-μL aliquot of extract was treated with color reagent in a disposable plastic microtiter plate (96 wells/plate), and color was developed by heating the plate in a low-temperature oven at 87°C for 55 min. After cooling for 20 min at ambient temperature, absorbance measurements were simultaneously performed on all 96 wells using a microplate reader. The microscale method was faster and more convenient than the conventional method; moreover, the volume of waste was markedly reduced. Studies to compare the two methods showed very little difference in accuracy, precision, or sensitivity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2519-2529 |
| Number of pages | 11 |
| Journal | Communications in Soil Science and Plant Analysis |
| Volume | 26 |
| Issue number | 15-16 |
| DOIs | |
| State | Published - Sep 1 1995 |
ASJC Scopus subject areas
- Agronomy and Crop Science
- Soil Science