A MicroRNA-Based Gene-Targeting Tool for Virally Labeling Interneurons in the Rodent Cortex

Marianna K. Keaveney, Hua an Tseng, Tina L. Ta, Howard J. Gritton, Heng Ye Man, Xue Han

Research output: Contribution to journalArticlepeer-review

Abstract

More specific and broadly applicable viral gene-targeting tools for labeling neuron subtypes are needed to advance neuroscience research, especially in rodent transgenic disease models and genetically intractable species. Here, we develop a viral vector that restricts transgene expression to GABAergic interneurons in the rodent neocortex by exploiting endogenous microRNA regulation. Our interneuron-targeting, microRNA-guided neuron tag, “GABA mAGNET,” achieves >95% interneuron selective labeling in the mouse cortex, including in a murine model of autism and also some preferential labeling of interneurons in the rat brain. We demonstrate an application of our GABA mAGNET by performing simultaneous, in vivo optogenetic control of two distinct neuron subtypes. This interneuron labeling tool highlights the potential of microRNA-based viral gene targeting to specific neuron subtypes.

Original languageEnglish (US)
Pages (from-to)294-303
Number of pages10
JournalCell Reports
Volume24
Issue number2
DOIs
StatePublished - Jul 10 2018
Externally publishedYes

Keywords

  • AAV
  • autism
  • GABAergic
  • gene targeting
  • gene therapy
  • genetic labeling
  • interneurons
  • lentivirus
  • mAGNET
  • microRNA
  • mouse
  • rat

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint

Dive into the research topics of 'A MicroRNA-Based Gene-Targeting Tool for Virally Labeling Interneurons in the Rodent Cortex'. Together they form a unique fingerprint.

Cite this