A microfluidic biochip platform for electrical quantification of proteins

Enrique Valera, Jacob Berger, Umer Hassan, Tanmay Ghonge, Julia Liu, Michael Rappleye, Jackson Winter, Daniel Abboud, Zeeshan Haidry, Ryan Healey, Na Teng Hung, Nathaniel Leung, Naif Mansury, Alexander Hasnain, Christine Lannon, Zachary Price, Karen White, Rashid Bashir

Research output: Contribution to journalArticle

Abstract

Sepsis, an adverse auto-immune response to an infection often causing life-threatening complications, results in the highest mortality and treatment cost of any illness in US hospitals. Several immune biomarker levels, including Interleukin 6 (IL-6), have shown a high correlation to the onset and progression of sepsis. Currently, no technology diagnoses and stratifies sepsis progression using biomarker levels. This paper reports a microfluidic biochip platform to detect proteins in undiluted human plasma samples. The device uses a differential enumeration platform that integrates Coulter counting principles, antigen specific capture chambers, and micro size bead based immunodetection to quantify cytokines. This microfluidic biochip was validated as a potential point of care technology by quantifying IL-6 from plasma samples (n = 29) with good correlation (R2 = 0.81) and agreement (Bland-Altman) compared to controls. In combination with previous applications, this point of care platform can potentially detect cell and protein biomarkers simultaneously for sepsis stratification.

Original languageEnglish (US)
Pages (from-to)1461-1470
Number of pages10
JournalLab on a chip
Volume18
Issue number10
DOIs
StatePublished - Jan 1 2018

Fingerprint

Biochips
Microfluidics
Biomarkers
Sepsis
Point-of-Care Systems
Proteins
Interleukin-6
Plasma (human)
Antigens
Cost of Illness
Health Care Costs
Cytokines
Plasmas
Technology
Equipment and Supplies
Mortality
Costs
Infection

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Valera, E., Berger, J., Hassan, U., Ghonge, T., Liu, J., Rappleye, M., ... Bashir, R. (2018). A microfluidic biochip platform for electrical quantification of proteins. Lab on a chip, 18(10), 1461-1470. https://doi.org/10.1039/c8lc00033f

A microfluidic biochip platform for electrical quantification of proteins. / Valera, Enrique; Berger, Jacob; Hassan, Umer; Ghonge, Tanmay; Liu, Julia; Rappleye, Michael; Winter, Jackson; Abboud, Daniel; Haidry, Zeeshan; Healey, Ryan; Hung, Na Teng; Leung, Nathaniel; Mansury, Naif; Hasnain, Alexander; Lannon, Christine; Price, Zachary; White, Karen; Bashir, Rashid.

In: Lab on a chip, Vol. 18, No. 10, 01.01.2018, p. 1461-1470.

Research output: Contribution to journalArticle

Valera, E, Berger, J, Hassan, U, Ghonge, T, Liu, J, Rappleye, M, Winter, J, Abboud, D, Haidry, Z, Healey, R, Hung, NT, Leung, N, Mansury, N, Hasnain, A, Lannon, C, Price, Z, White, K & Bashir, R 2018, 'A microfluidic biochip platform for electrical quantification of proteins', Lab on a chip, vol. 18, no. 10, pp. 1461-1470. https://doi.org/10.1039/c8lc00033f
Valera E, Berger J, Hassan U, Ghonge T, Liu J, Rappleye M et al. A microfluidic biochip platform for electrical quantification of proteins. Lab on a chip. 2018 Jan 1;18(10):1461-1470. https://doi.org/10.1039/c8lc00033f
Valera, Enrique ; Berger, Jacob ; Hassan, Umer ; Ghonge, Tanmay ; Liu, Julia ; Rappleye, Michael ; Winter, Jackson ; Abboud, Daniel ; Haidry, Zeeshan ; Healey, Ryan ; Hung, Na Teng ; Leung, Nathaniel ; Mansury, Naif ; Hasnain, Alexander ; Lannon, Christine ; Price, Zachary ; White, Karen ; Bashir, Rashid. / A microfluidic biochip platform for electrical quantification of proteins. In: Lab on a chip. 2018 ; Vol. 18, No. 10. pp. 1461-1470.
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