A microfluidic approach for protein structure determination at room temperature via on-chip anomalous diffraction

Sarah L. Perry; Sudipto Guha; Ashtamurthy S. Pawate; Amrit Bhaskarla; Vinayak Agarwal; Satish K. Nair; Paul J.A. Kenis

doi:10.1039/c3lc50276g

A microfluidic approach for protein structure determination at room temperature via on-chip anomalous diffraction

Sarah L. Perry, Sudipto Guha, Ashtamurthy S. Pawate, Amrit Bhaskarla, Vinayak Agarwal, Satish K. Nair, Paul J.A. Kenis

Research output: Contribution to journal › Article › peer-review

Abstract

We report a microfluidic approach for de novo protein structure determination via crystallization screening and optimization, as well as on-chip X-ray diffraction data collection. The structure of phosphonoacetate hydrolase (PhnA) has been solved to 2.11 Å via on-chip collection of anomalous data that has an order of magnitude lower mosaicity than what is typical for traditional structure determination methods.

Original language	English (US)
Pages (from-to)	3183-3187
Number of pages	5
Journal	Lab on a chip
Volume	13
Issue number	16
DOIs	https://doi.org/10.1039/c3lc50276g
State	Published - Aug 21 2013

ASJC Scopus subject areas

Bioengineering
Biochemistry
General Chemistry
Biomedical Engineering

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10.1039/c3lc50276g

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AU - Bhaskarla, Amrit

AU - Agarwal, Vinayak

AU - Nair, Satish K.

AU - Kenis, Paul J.A.

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AB - We report a microfluidic approach for de novo protein structure determination via crystallization screening and optimization, as well as on-chip X-ray diffraction data collection. The structure of phosphonoacetate hydrolase (PhnA) has been solved to 2.11 Å via on-chip collection of anomalous data that has an order of magnitude lower mosaicity than what is typical for traditional structure determination methods.

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A microfluidic approach for protein structure determination at room temperature via on-chip anomalous diffraction

Abstract

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