Abstract
We report a microfluidic approach for de novo protein structure determination via crystallization screening and optimization, as well as on-chip X-ray diffraction data collection. The structure of phosphonoacetate hydrolase (PhnA) has been solved to 2.11 Å via on-chip collection of anomalous data that has an order of magnitude lower mosaicity than what is typical for traditional structure determination methods.
Original language | English (US) |
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Pages (from-to) | 3183-3187 |
Number of pages | 5 |
Journal | Lab on a chip |
Volume | 13 |
Issue number | 16 |
DOIs | |
State | Published - Aug 21 2013 |
ASJC Scopus subject areas
- Bioengineering
- Biochemistry
- General Chemistry
- Biomedical Engineering