A microfluidic approach for protein structure determination at room temperature via on-chip anomalous diffraction

Sarah L. Perry, Sudipto Guha, Ashtamurthy S. Pawate, Amrit Bhaskarla, Vinayak Agarwal, Satish K. Nair, Paul J.A. Kenis

Research output: Contribution to journalArticlepeer-review

Abstract

We report a microfluidic approach for de novo protein structure determination via crystallization screening and optimization, as well as on-chip X-ray diffraction data collection. The structure of phosphonoacetate hydrolase (PhnA) has been solved to 2.11 Å via on-chip collection of anomalous data that has an order of magnitude lower mosaicity than what is typical for traditional structure determination methods.

Original languageEnglish (US)
Pages (from-to)3183-3187
Number of pages5
JournalLab on a chip
Volume13
Issue number16
DOIs
StatePublished - Aug 21 2013

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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