A microfabricated electrical differential counter for the selective enumeration of CD4+ T lymphocytes

Nicholas N. Watkins, Supriya Sridhar, Xuanhong Cheng, Grace D. Chen, Mehmet Toner, William Rodriguez, Rashid Bashir

Research output: Contribution to journalArticle

Abstract

We have developed a microfabricated biochip that enumerates CD4+ T lymphocytes from leukocyte populations obtained from human blood samples using electrical impedance sensing and immunoaffinity chromatography. T cell counts are found by obtaining the difference between the number of leukocytes before and after depleting CD4+ T cells with immobilized antibodies in a capture chamber. This differential counting technique has been validated to analyze physiological concentrations of leukocytes with an accuracy of ∼9 cells per L by passivating the capture chamber with bovine serum albumin. In addition, the counter provided T cell counts which correlated closely with an optical control (R2 = 0.997) for CD4 cell concentrations ranging from approximately 100 to 700 cells per L. We believe that this approach can be a promising method for bringing quantitative HIV/AIDS diagnostics to resource-poor regions in the world.

Original languageEnglish (US)
Pages (from-to)1437-1447
Number of pages11
JournalLab on a chip
Volume11
Issue number8
DOIs
StatePublished - Apr 21 2011

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T-cells
T-Lymphocytes
Leukocytes
Cell Count
Immobilized Antibodies
Biochips
Acoustic impedance
Bovine Serum Albumin
Chromatography
Electric Impedance
Leukocyte Count
Antibodies
Acquired Immunodeficiency Syndrome
Blood
HIV
Population

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Watkins, N. N., Sridhar, S., Cheng, X., Chen, G. D., Toner, M., Rodriguez, W., & Bashir, R. (2011). A microfabricated electrical differential counter for the selective enumeration of CD4+ T lymphocytes. Lab on a chip, 11(8), 1437-1447. https://doi.org/10.1039/c0lc00556h

A microfabricated electrical differential counter for the selective enumeration of CD4+ T lymphocytes. / Watkins, Nicholas N.; Sridhar, Supriya; Cheng, Xuanhong; Chen, Grace D.; Toner, Mehmet; Rodriguez, William; Bashir, Rashid.

In: Lab on a chip, Vol. 11, No. 8, 21.04.2011, p. 1437-1447.

Research output: Contribution to journalArticle

Watkins, NN, Sridhar, S, Cheng, X, Chen, GD, Toner, M, Rodriguez, W & Bashir, R 2011, 'A microfabricated electrical differential counter for the selective enumeration of CD4+ T lymphocytes', Lab on a chip, vol. 11, no. 8, pp. 1437-1447. https://doi.org/10.1039/c0lc00556h
Watkins NN, Sridhar S, Cheng X, Chen GD, Toner M, Rodriguez W et al. A microfabricated electrical differential counter for the selective enumeration of CD4+ T lymphocytes. Lab on a chip. 2011 Apr 21;11(8):1437-1447. https://doi.org/10.1039/c0lc00556h
Watkins, Nicholas N. ; Sridhar, Supriya ; Cheng, Xuanhong ; Chen, Grace D. ; Toner, Mehmet ; Rodriguez, William ; Bashir, Rashid. / A microfabricated electrical differential counter for the selective enumeration of CD4+ T lymphocytes. In: Lab on a chip. 2011 ; Vol. 11, No. 8. pp. 1437-1447.
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