A lophotrochozoan-specific nuclear hormone receptor is required for reproductive system development in the planarian

Marla E. Tharp, James J. Collins, Phillip A. Newmark

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Germ cells of sexually reproducing organisms receive an array of cues from somatic tissues that instruct developmental processes. Although the nature of these signals differs amongst organisms, the importance of germline-soma interactions is a common theme. Recently, peptide hormones from the nervous system have been shown to regulate germ cell development in the planarian Schmidtea mediterranea; thus, we sought to investigate a second class of hormones with a conserved role in reproduction, the lipophilic hormones. In order to study these signals, we identified a set of putative lipophilic hormone receptors, known as nuclear hormone receptors, and analyzed their functions in reproductive development. We found one gene, nhr-1, belonging to a small class of functionally uncharacterized lophotrochozoan-specific receptors, to be essential for the development of differentiated germ cells. Upon nhr-1 knockdown, germ cells in the testes and ovaries fail to mature, and remain as undifferentiated germline stem cells. Further analysis revealed that nhr-1 mRNA is expressed in the accessory reproductive organs and is required for their development, suggesting that this transcription factor functions cell non-autonomously in regulating germ cell development. Our studies identify a role for nuclear hormone receptors in planarian reproductive maturation and reinforce the significance of germline-soma interactions in sexual reproduction across metazoans.

    Original languageEnglish (US)
    Pages (from-to)150-157
    Number of pages8
    JournalDevelopmental Biology
    Volume396
    Issue number1
    DOIs
    StatePublished - Dec 1 2014

    Keywords

    • Accessory reproductive organ
    • Germ cell
    • Lophotrochozoa
    • Nuclear hormone receptor
    • Planaria

    ASJC Scopus subject areas

    • Molecular Biology
    • Developmental Biology
    • Cell Biology

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