Abstract

Low-cost detection of pathogens and biomolecules at the point-of-care promises to revolutionize medicine through more individualized monitoring and increased accessibility to diagnostics in remote and resource-limited areas. While many approaches to biosensing are still limited by expensive components or inadequate portability, we present here an ELISA-inspired lab-on-a-chip strategy for biological detection based on liposome tagging and ion-release impedance spectroscopy. Ion-encapsulating dipalmitoylphosphatidylcholine (DPPC) liposomes can be functionalized with antibodies and are stable in deionized water yet permeabilized for ion release upon heating, making them ideal reporters for electrical biosensing of surface-immobilized antigens. We demonstrate the quantification of these liposomes by real-time impedance measurements, as well as the qualitative detection of viruses as a proof-of-concept toward a portable platform for viral load determination which can be applied broadly to the detection of pathogens and other biomolecules.

Original languageEnglish (US)
Pages (from-to)895-905
Number of pages11
JournalBiomedical microdevices
Volume15
Issue number5
DOIs
StatePublished - Oct 1 2013

Fingerprint

Liposomes
Electric Impedance
Biomolecules
Pathogens
Ions
Sensors
Point-of-Care Systems
Dielectric Spectroscopy
1,2-Dipalmitoylphosphatidylcholine
Lab-on-a-chip
Deionized water
Surface Antigens
Antigens
Viral Load
Viruses
Antibodies
Heating
Medicine
Enzyme-Linked Immunosorbent Assay
Spectroscopy

Keywords

  • Impedance sensing
  • Liposomes
  • Microfluidics
  • Point-of-care

ASJC Scopus subject areas

  • Biomedical Engineering
  • Molecular Biology

Cite this

A liposome-based ion release impedance sensor for biological detection. / Damhorst, Gregory L.; Smith, Cartney E.; Salm, Eric M.; Sobieraj, Magdalena M.; Ni, Hengkan; Kong, Hyunjoon; Bashir, Rashid.

In: Biomedical microdevices, Vol. 15, No. 5, 01.10.2013, p. 895-905.

Research output: Contribution to journalArticle

Damhorst, Gregory L. ; Smith, Cartney E. ; Salm, Eric M. ; Sobieraj, Magdalena M. ; Ni, Hengkan ; Kong, Hyunjoon ; Bashir, Rashid. / A liposome-based ion release impedance sensor for biological detection. In: Biomedical microdevices. 2013 ; Vol. 15, No. 5. pp. 895-905.
@article{dac3e3622ae843f0bc9cb895e58faa8f,
title = "A liposome-based ion release impedance sensor for biological detection",
abstract = "Low-cost detection of pathogens and biomolecules at the point-of-care promises to revolutionize medicine through more individualized monitoring and increased accessibility to diagnostics in remote and resource-limited areas. While many approaches to biosensing are still limited by expensive components or inadequate portability, we present here an ELISA-inspired lab-on-a-chip strategy for biological detection based on liposome tagging and ion-release impedance spectroscopy. Ion-encapsulating dipalmitoylphosphatidylcholine (DPPC) liposomes can be functionalized with antibodies and are stable in deionized water yet permeabilized for ion release upon heating, making them ideal reporters for electrical biosensing of surface-immobilized antigens. We demonstrate the quantification of these liposomes by real-time impedance measurements, as well as the qualitative detection of viruses as a proof-of-concept toward a portable platform for viral load determination which can be applied broadly to the detection of pathogens and other biomolecules.",
keywords = "Impedance sensing, Liposomes, Microfluidics, Point-of-care",
author = "Damhorst, {Gregory L.} and Smith, {Cartney E.} and Salm, {Eric M.} and Sobieraj, {Magdalena M.} and Hengkan Ni and Hyunjoon Kong and Rashid Bashir",
year = "2013",
month = "10",
day = "1",
doi = "10.1007/s10544-013-9778-4",
language = "English (US)",
volume = "15",
pages = "895--905",
journal = "Biomedical Microdevices",
issn = "1387-2176",
publisher = "Kluwer Academic Publishers",
number = "5",

}

TY - JOUR

T1 - A liposome-based ion release impedance sensor for biological detection

AU - Damhorst, Gregory L.

AU - Smith, Cartney E.

AU - Salm, Eric M.

AU - Sobieraj, Magdalena M.

AU - Ni, Hengkan

AU - Kong, Hyunjoon

AU - Bashir, Rashid

PY - 2013/10/1

Y1 - 2013/10/1

N2 - Low-cost detection of pathogens and biomolecules at the point-of-care promises to revolutionize medicine through more individualized monitoring and increased accessibility to diagnostics in remote and resource-limited areas. While many approaches to biosensing are still limited by expensive components or inadequate portability, we present here an ELISA-inspired lab-on-a-chip strategy for biological detection based on liposome tagging and ion-release impedance spectroscopy. Ion-encapsulating dipalmitoylphosphatidylcholine (DPPC) liposomes can be functionalized with antibodies and are stable in deionized water yet permeabilized for ion release upon heating, making them ideal reporters for electrical biosensing of surface-immobilized antigens. We demonstrate the quantification of these liposomes by real-time impedance measurements, as well as the qualitative detection of viruses as a proof-of-concept toward a portable platform for viral load determination which can be applied broadly to the detection of pathogens and other biomolecules.

AB - Low-cost detection of pathogens and biomolecules at the point-of-care promises to revolutionize medicine through more individualized monitoring and increased accessibility to diagnostics in remote and resource-limited areas. While many approaches to biosensing are still limited by expensive components or inadequate portability, we present here an ELISA-inspired lab-on-a-chip strategy for biological detection based on liposome tagging and ion-release impedance spectroscopy. Ion-encapsulating dipalmitoylphosphatidylcholine (DPPC) liposomes can be functionalized with antibodies and are stable in deionized water yet permeabilized for ion release upon heating, making them ideal reporters for electrical biosensing of surface-immobilized antigens. We demonstrate the quantification of these liposomes by real-time impedance measurements, as well as the qualitative detection of viruses as a proof-of-concept toward a portable platform for viral load determination which can be applied broadly to the detection of pathogens and other biomolecules.

KW - Impedance sensing

KW - Liposomes

KW - Microfluidics

KW - Point-of-care

UR - http://www.scopus.com/inward/record.url?scp=84884352926&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84884352926&partnerID=8YFLogxK

U2 - 10.1007/s10544-013-9778-4

DO - 10.1007/s10544-013-9778-4

M3 - Article

C2 - 23793417

AN - SCOPUS:84884352926

VL - 15

SP - 895

EP - 905

JO - Biomedical Microdevices

JF - Biomedical Microdevices

SN - 1387-2176

IS - 5

ER -