A liposome-based ion release impedance sensor for biological detection

Gregory L. Damhorst; Cartney E. Smith; Eric M. Salm; Magdalena M. Sobieraj; Hengkan Ni; Hyunjoon Kong; Rashid Bashir

doi:10.1007/s10544-013-9778-4

A liposome-based ion release impedance sensor for biological detection

Gregory L. Damhorst, Cartney E. Smith, Eric M. Salm, Magdalena M. Sobieraj, Hengkan Ni, Hyunjoon Kong, Rashid Bashir

Research output: Contribution to journal › Article › peer-review

Abstract

Low-cost detection of pathogens and biomolecules at the point-of-care promises to revolutionize medicine through more individualized monitoring and increased accessibility to diagnostics in remote and resource-limited areas. While many approaches to biosensing are still limited by expensive components or inadequate portability, we present here an ELISA-inspired lab-on-a-chip strategy for biological detection based on liposome tagging and ion-release impedance spectroscopy. Ion-encapsulating dipalmitoylphosphatidylcholine (DPPC) liposomes can be functionalized with antibodies and are stable in deionized water yet permeabilized for ion release upon heating, making them ideal reporters for electrical biosensing of surface-immobilized antigens. We demonstrate the quantification of these liposomes by real-time impedance measurements, as well as the qualitative detection of viruses as a proof-of-concept toward a portable platform for viral load determination which can be applied broadly to the detection of pathogens and other biomolecules.

Original language	English (US)
Pages (from-to)	895-905
Number of pages	11
Journal	Biomedical microdevices
Volume	15
Issue number	5
DOIs	https://doi.org/10.1007/s10544-013-9778-4
State	Published - Oct 2013

Keywords

Impedance sensing
Liposomes
Microfluidics
Point-of-care

ASJC Scopus subject areas

Biomedical Engineering
Molecular Biology

Online availability

10.1007/s10544-013-9778-4

Library availability

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title = "A liposome-based ion release impedance sensor for biological detection",

abstract = "Low-cost detection of pathogens and biomolecules at the point-of-care promises to revolutionize medicine through more individualized monitoring and increased accessibility to diagnostics in remote and resource-limited areas. While many approaches to biosensing are still limited by expensive components or inadequate portability, we present here an ELISA-inspired lab-on-a-chip strategy for biological detection based on liposome tagging and ion-release impedance spectroscopy. Ion-encapsulating dipalmitoylphosphatidylcholine (DPPC) liposomes can be functionalized with antibodies and are stable in deionized water yet permeabilized for ion release upon heating, making them ideal reporters for electrical biosensing of surface-immobilized antigens. We demonstrate the quantification of these liposomes by real-time impedance measurements, as well as the qualitative detection of viruses as a proof-of-concept toward a portable platform for viral load determination which can be applied broadly to the detection of pathogens and other biomolecules.",

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author = "Damhorst, {Gregory L.} and Smith, {Cartney E.} and Salm, {Eric M.} and Sobieraj, {Magdalena M.} and Hengkan Ni and Hyunjoon Kong and Rashid Bashir",

note = "Funding Information: Acknowledgments We would like to thank Mehmet Toner at Massachusetts General Hospital, William Rodriguez and Marta Fernandez Suarez at Daktari Diagnostics, Inc., and Joshua Wood and Brian Dorvel at the University of Illinois for helpful discussions. Partial support was provided by the Illinois Distinguished Fellowship (to GLD) at the University of Illinois at Urbana-Champaign. This work was also supported by the National Institutes of Health (1R01 HL109192 to H.J.K. and Chemistry-Biology Interface Training Program 5T32GM070421 to C.E.S.). The devices were fabricated and tested at the Micro and Nanotechnology Laboratory at the University of Illinois at Urbana-Champaign (www.mntl.illinois.edu).",

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AU - Smith, Cartney E.

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AU - Ni, Hengkan

AU - Kong, Hyunjoon

AU - Bashir, Rashid

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N2 - Low-cost detection of pathogens and biomolecules at the point-of-care promises to revolutionize medicine through more individualized monitoring and increased accessibility to diagnostics in remote and resource-limited areas. While many approaches to biosensing are still limited by expensive components or inadequate portability, we present here an ELISA-inspired lab-on-a-chip strategy for biological detection based on liposome tagging and ion-release impedance spectroscopy. Ion-encapsulating dipalmitoylphosphatidylcholine (DPPC) liposomes can be functionalized with antibodies and are stable in deionized water yet permeabilized for ion release upon heating, making them ideal reporters for electrical biosensing of surface-immobilized antigens. We demonstrate the quantification of these liposomes by real-time impedance measurements, as well as the qualitative detection of viruses as a proof-of-concept toward a portable platform for viral load determination which can be applied broadly to the detection of pathogens and other biomolecules.

AB - Low-cost detection of pathogens and biomolecules at the point-of-care promises to revolutionize medicine through more individualized monitoring and increased accessibility to diagnostics in remote and resource-limited areas. While many approaches to biosensing are still limited by expensive components or inadequate portability, we present here an ELISA-inspired lab-on-a-chip strategy for biological detection based on liposome tagging and ion-release impedance spectroscopy. Ion-encapsulating dipalmitoylphosphatidylcholine (DPPC) liposomes can be functionalized with antibodies and are stable in deionized water yet permeabilized for ion release upon heating, making them ideal reporters for electrical biosensing of surface-immobilized antigens. We demonstrate the quantification of these liposomes by real-time impedance measurements, as well as the qualitative detection of viruses as a proof-of-concept toward a portable platform for viral load determination which can be applied broadly to the detection of pathogens and other biomolecules.

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A liposome-based ion release impedance sensor for biological detection

Abstract

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