Abstract
Rapid eye movement (REM) and non-REM (NREM) sleep are controlled by specific neuronal circuits. Here we show that galanin-expressing GABAergic neurons in the dorsomedial hypothalamus (DMH) comprise separate subpopulations with opposing effects on REM versus NREM sleep. Microendoscopic calcium imaging revealed diverse sleep-wake activity of DMH GABAergic neurons, but the galanin-expressing subset falls into two distinct groups, either selectively activated (REM-on) or suppressed (REM-off) during REM sleep. Retrogradely labeled, preoptic area (POA)-projecting galaninergic neurons are REM-off, whereas the raphe pallidus (RPA)-projecting neurons are primarily REM-on. Bidirectional optogenetic manipulations showed that the POA-projecting neurons promote NREM sleep and suppress REM sleep, while the RPA-projecting neurons have the opposite effects. Thus, REM/NREM switch is regulated antagonistically by DMH galaninergic neurons with intermingled cell bodies but distinct axon projections. Using microendoscopic calcium imaging and bidirectional optogenetic manipulation, Chen et al. showed that galaninergic neurons in the dorsomedial hypothalamus consist of two subpopulations with distinct axon projections and opposing roles in regulating the switch between REM and non-REM sleep.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1168-1176.e4 |
| Journal | Neuron |
| Volume | 97 |
| Issue number | 5 |
| DOIs | |
| State | Published - Mar 7 2018 |
| Externally published | Yes |
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Keywords
- dorsomedial hypothalamus
- galanin
- sleep
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
A Hypothalamic Switch for REM and Non-REM Sleep. / Chen, Kai Siang; Xu, Min; Zhang, Zhe; Chang, Wei Cheng; Gaj, Thomas; Schaffer, David V.; Dan, Yang.
In: Neuron, Vol. 97, No. 5, 07.03.2018, p. 1168-1176.e4.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A Hypothalamic Switch for REM and Non-REM Sleep
AU - Chen, Kai Siang
AU - Xu, Min
AU - Zhang, Zhe
AU - Chang, Wei Cheng
AU - Gaj, Thomas
AU - Schaffer, David V.
AU - Dan, Yang
PY - 2018/3/7
Y1 - 2018/3/7
N2 - Rapid eye movement (REM) and non-REM (NREM) sleep are controlled by specific neuronal circuits. Here we show that galanin-expressing GABAergic neurons in the dorsomedial hypothalamus (DMH) comprise separate subpopulations with opposing effects on REM versus NREM sleep. Microendoscopic calcium imaging revealed diverse sleep-wake activity of DMH GABAergic neurons, but the galanin-expressing subset falls into two distinct groups, either selectively activated (REM-on) or suppressed (REM-off) during REM sleep. Retrogradely labeled, preoptic area (POA)-projecting galaninergic neurons are REM-off, whereas the raphe pallidus (RPA)-projecting neurons are primarily REM-on. Bidirectional optogenetic manipulations showed that the POA-projecting neurons promote NREM sleep and suppress REM sleep, while the RPA-projecting neurons have the opposite effects. Thus, REM/NREM switch is regulated antagonistically by DMH galaninergic neurons with intermingled cell bodies but distinct axon projections. Using microendoscopic calcium imaging and bidirectional optogenetic manipulation, Chen et al. showed that galaninergic neurons in the dorsomedial hypothalamus consist of two subpopulations with distinct axon projections and opposing roles in regulating the switch between REM and non-REM sleep.
AB - Rapid eye movement (REM) and non-REM (NREM) sleep are controlled by specific neuronal circuits. Here we show that galanin-expressing GABAergic neurons in the dorsomedial hypothalamus (DMH) comprise separate subpopulations with opposing effects on REM versus NREM sleep. Microendoscopic calcium imaging revealed diverse sleep-wake activity of DMH GABAergic neurons, but the galanin-expressing subset falls into two distinct groups, either selectively activated (REM-on) or suppressed (REM-off) during REM sleep. Retrogradely labeled, preoptic area (POA)-projecting galaninergic neurons are REM-off, whereas the raphe pallidus (RPA)-projecting neurons are primarily REM-on. Bidirectional optogenetic manipulations showed that the POA-projecting neurons promote NREM sleep and suppress REM sleep, while the RPA-projecting neurons have the opposite effects. Thus, REM/NREM switch is regulated antagonistically by DMH galaninergic neurons with intermingled cell bodies but distinct axon projections. Using microendoscopic calcium imaging and bidirectional optogenetic manipulation, Chen et al. showed that galaninergic neurons in the dorsomedial hypothalamus consist of two subpopulations with distinct axon projections and opposing roles in regulating the switch between REM and non-REM sleep.
KW - dorsomedial hypothalamus
KW - galanin
KW - sleep
UR - http://www.scopus.com/inward/record.url?scp=85042633791&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85042633791&partnerID=8YFLogxK
U2 - 10.1016/j.neuron.2018.02.005
DO - 10.1016/j.neuron.2018.02.005
M3 - Article
C2 - 29478915
AN - SCOPUS:85042633791
VL - 97
SP - 1168-1176.e4
JO - Neuron
JF - Neuron
SN - 0896-6273
IS - 5
ER -