A Hypothalamic Switch for REM and Non-REM Sleep

Kai Siang Chen, Min Xu, Zhe Zhang, Wei Cheng Chang, Thomas Gaj, David V. Schaffer, Yang Dan

Research output: Contribution to journalArticle

Abstract

Rapid eye movement (REM) and non-REM (NREM) sleep are controlled by specific neuronal circuits. Here we show that galanin-expressing GABAergic neurons in the dorsomedial hypothalamus (DMH) comprise separate subpopulations with opposing effects on REM versus NREM sleep. Microendoscopic calcium imaging revealed diverse sleep-wake activity of DMH GABAergic neurons, but the galanin-expressing subset falls into two distinct groups, either selectively activated (REM-on) or suppressed (REM-off) during REM sleep. Retrogradely labeled, preoptic area (POA)-projecting galaninergic neurons are REM-off, whereas the raphe pallidus (RPA)-projecting neurons are primarily REM-on. Bidirectional optogenetic manipulations showed that the POA-projecting neurons promote NREM sleep and suppress REM sleep, while the RPA-projecting neurons have the opposite effects. Thus, REM/NREM switch is regulated antagonistically by DMH galaninergic neurons with intermingled cell bodies but distinct axon projections. Using microendoscopic calcium imaging and bidirectional optogenetic manipulation, Chen et al. showed that galaninergic neurons in the dorsomedial hypothalamus consist of two subpopulations with distinct axon projections and opposing roles in regulating the switch between REM and non-REM sleep.

Original languageEnglish (US)
Pages (from-to)1168-1176.e4
JournalNeuron
Volume97
Issue number5
DOIs
StatePublished - Mar 7 2018
Externally publishedYes

Fingerprint

REM Sleep
Eye Movements
Sleep
Neurons
Hypothalamus
Optogenetics
Galanin
GABAergic Neurons
Preoptic Area
Axons
Calcium

Keywords

  • dorsomedial hypothalamus
  • galanin
  • sleep

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Chen, K. S., Xu, M., Zhang, Z., Chang, W. C., Gaj, T., Schaffer, D. V., & Dan, Y. (2018). A Hypothalamic Switch for REM and Non-REM Sleep. Neuron, 97(5), 1168-1176.e4. https://doi.org/10.1016/j.neuron.2018.02.005

A Hypothalamic Switch for REM and Non-REM Sleep. / Chen, Kai Siang; Xu, Min; Zhang, Zhe; Chang, Wei Cheng; Gaj, Thomas; Schaffer, David V.; Dan, Yang.

In: Neuron, Vol. 97, No. 5, 07.03.2018, p. 1168-1176.e4.

Research output: Contribution to journalArticle

Chen, KS, Xu, M, Zhang, Z, Chang, WC, Gaj, T, Schaffer, DV & Dan, Y 2018, 'A Hypothalamic Switch for REM and Non-REM Sleep', Neuron, vol. 97, no. 5, pp. 1168-1176.e4. https://doi.org/10.1016/j.neuron.2018.02.005
Chen KS, Xu M, Zhang Z, Chang WC, Gaj T, Schaffer DV et al. A Hypothalamic Switch for REM and Non-REM Sleep. Neuron. 2018 Mar 7;97(5):1168-1176.e4. https://doi.org/10.1016/j.neuron.2018.02.005
Chen, Kai Siang ; Xu, Min ; Zhang, Zhe ; Chang, Wei Cheng ; Gaj, Thomas ; Schaffer, David V. ; Dan, Yang. / A Hypothalamic Switch for REM and Non-REM Sleep. In: Neuron. 2018 ; Vol. 97, No. 5. pp. 1168-1176.e4.
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