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A highly sensitive selection method for directed evolution of homing endonucleases
Zhilei Chen,
Huimin Zhao
Chemical and Biomolecular Engineering
Bioengineering
Biomedical and Translational Sciences
Chemistry
Carl R. Woese Institute for Genomic Biology
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peer-review
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Keyphrases
Highly Sensitive
100%
Directed Evolution
100%
Selection Strategy
100%
Homing Endonuclease
100%
I-SceI
80%
DNA Cleavage
40%
DNA Sequencing
20%
Gene-gene
20%
Sequence Specificity
20%
Targeted Sequencing
20%
Metabolic Engineering
20%
Systems Biology
20%
Survival Rate
20%
Homologous Recombination
20%
Host Cell
20%
Double-strand Breaks
20%
OR Genes
20%
Gene Therapy
20%
Recognition Site
20%
Cell Survival Rate
20%
Gene Alteration
20%
Gene Correction
20%
Biochemistry, Genetics and Molecular Biology
Homing Endonuclease
100%
Enzyme
60%
DNA Cleavage
40%
Survival Rate
40%
Metabolic Engineering
20%
DNA Sequence
20%
Wild Type
20%
Homologous Recombination
20%
Gene Therapy
20%
Cell Survival
20%
Gene Mutation
20%