A High-Efficiency HeLa Cell Nuclear Transcription Extract

David J. Shapiro, Phillip A. Sharp, Walter W. Wahli, Martha J. Keller

Research output: Contribution to journalArticlepeer-review


A HeLa cell nuclear transcription extract that is approximately 20 times more efficient than standard Hela cell transcription extracts was developed. Transcription of the strong adenovirus II major late promoter by this extract results in the synthesis of 1.5–4 molecules of product RNA per molecule of template, indicating that the extract is capable of multiple rounds of initiation. Standard HeLa cell nuclear extracts transcribe closed circular and linear adenovirus major late promoter templates with equal efficiency. In contrast, the new extract exhibits an increase of approximately twofold on transcription of a closed circular, as opposed to a linear, major late promoter template.

Original languageEnglish (US)
Pages (from-to)47-55
Number of pages9
Issue number1
StatePublished - Jan 1988

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics


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