TY - JOUR
T1 - A genetic linkage map of the soybean cyst nematode Heterodera glycines
AU - Atibalentja, N.
AU - Bekal, S.
AU - Domier, L. L.
AU - Niblack, T. L.
AU - Noel, G. R.
AU - Lambert, K. N.
N1 - Funding Information:
Acknowledgements This study was supported by grants from the Illinois Soybean Program Operating Board (ISPOB), the North Central Soybean Research Program (NCSRP), the Soybean Disease Biotechnology Center (SDBC) at the University of Illinois at Urbana-Champaign, and the United Soybean Board (USB). Linkage analyses were conducted in the Computer Laboratory of Dr. T.R. Rocheford, Professor of Crop Sciences at the University of Illinois at Urbana-Champaign. We also wish to thank K. Col-grove, A. Colgrove, R. Heinz, and K. Hajsaid for their technical support. Mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval to the exclusion of other products that may also be suitable. This work has been carried out in compliance with the current laws governing genetic experimentation in the USA
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2005/5
Y1 - 2005/5
N2 - A genetic linkage map of the soybean cyst nematode (SCN) Heterodera glycines was constructed using a population of F2 individuals obtained from matings between two highly inbred SCN lines, TN16 and TN20. The AFLP fingerprinting technique was used to genotype 63 F2 progeny with two restriction enzyme combinations (EcoRI/MseI and PstI/TaqI) and 38 primer combinations. The same F2 population was also genotyped for Hg-cm-1 (H. glycines chorismate mutase-1), a putative virulence gene, using real-time quantitative PCR. Some of the markers were found to be distributed non-randomly. Even so, of the 230 markers analyzed, 131 could be mapped onto ten linkage groups at a minimum LOD of 3.0, for a total map distance of 539 cM. The Hg-cm-1 locus mapped to linkage group III together with 16 other markers. The size of the H. glycines genome was estimated to be in the range of 630-743 cM, indicating that the current map represents 73-86% of the genome, with a marker density of one per 4.5 cM, and a physical/genetic distance ratio of between 124 kb/cM and 147 kb/cM. This genetic map will be of great assistance in mapping H. glycines markers to genes of interest, such as nematode virulence genes and genes that control aspects of nematode parasitism.
AB - A genetic linkage map of the soybean cyst nematode (SCN) Heterodera glycines was constructed using a population of F2 individuals obtained from matings between two highly inbred SCN lines, TN16 and TN20. The AFLP fingerprinting technique was used to genotype 63 F2 progeny with two restriction enzyme combinations (EcoRI/MseI and PstI/TaqI) and 38 primer combinations. The same F2 population was also genotyped for Hg-cm-1 (H. glycines chorismate mutase-1), a putative virulence gene, using real-time quantitative PCR. Some of the markers were found to be distributed non-randomly. Even so, of the 230 markers analyzed, 131 could be mapped onto ten linkage groups at a minimum LOD of 3.0, for a total map distance of 539 cM. The Hg-cm-1 locus mapped to linkage group III together with 16 other markers. The size of the H. glycines genome was estimated to be in the range of 630-743 cM, indicating that the current map represents 73-86% of the genome, with a marker density of one per 4.5 cM, and a physical/genetic distance ratio of between 124 kb/cM and 147 kb/cM. This genetic map will be of great assistance in mapping H. glycines markers to genes of interest, such as nematode virulence genes and genes that control aspects of nematode parasitism.
KW - AFLP markers
KW - Plant pathogen
KW - Real-time PCR
KW - Whole-genome amplification
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U2 - 10.1007/s00438-005-1125-x
DO - 10.1007/s00438-005-1125-x
M3 - Article
C2 - 15902493
AN - SCOPUS:20044374019
SN - 1617-4615
VL - 273
SP - 273
EP - 281
JO - Molecular Genetics and Genomics
JF - Molecular Genetics and Genomics
IS - 3
ER -