A general two-step strategy to synthesize lariat RNAs

Yangming Wang, Scott K. Silverman

Research output: Contribution to journalArticle

Abstract

We describe a general and efficient two-step strategy for lariat RNA synthesis. In the first step, a deoxyribozyme synthesizes 2′,5′- branched RNA. In the second step, T4 RNA ligase closes the loop that completes the lariat. The loop-closure reaction can form either a natural or unnatural lariat isomer, depending on which of the two 3′-termini of the branched RNA reacts with the lone 5′-end. We demonstrate two approaches to control formation of either lariat isomer. In conjunction with other routes for lariat RNA synthesis, the two-step strategy described here will facilitate biochemical studies that require lariat RNAs of varying nucleotide sequence. Published by Cold Spring Harbor Laboratory Press.

Original languageEnglish (US)
Pages (from-to)313-321
Number of pages9
JournalRNA
Volume12
Issue number2
DOIs
StatePublished - Feb 1 2006

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Keywords

  • Branched RNA
  • Debranching enzyme
  • Deoxyribozyme
  • Lariat RNA
  • T4 RNA ligase

ASJC Scopus subject areas

  • Molecular Biology

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