A general two-step strategy to synthesize lariat RNAs

Yangming Wang, Scott K Silverman

Research output: Contribution to journalArticle

Abstract

We describe a general and efficient two-step strategy for lariat RNA synthesis. In the first step, a deoxyribozyme synthesizes 2′,5′- branched RNA. In the second step, T4 RNA ligase closes the loop that completes the lariat. The loop-closure reaction can form either a natural or unnatural lariat isomer, depending on which of the two 3′-termini of the branched RNA reacts with the lone 5′-end. We demonstrate two approaches to control formation of either lariat isomer. In conjunction with other routes for lariat RNA synthesis, the two-step strategy described here will facilitate biochemical studies that require lariat RNAs of varying nucleotide sequence. Published by Cold Spring Harbor Laboratory Press.

Original languageEnglish (US)
Pages (from-to)313-321
Number of pages9
JournalRNA
Volume12
Issue number2
DOIs
StatePublished - Feb 1 2006

Fingerprint

RNA
Catalytic DNA
Ligases

Keywords

  • Branched RNA
  • Debranching enzyme
  • Deoxyribozyme
  • Lariat RNA
  • T4 RNA ligase

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

A general two-step strategy to synthesize lariat RNAs. / Wang, Yangming; Silverman, Scott K.

In: RNA, Vol. 12, No. 2, 01.02.2006, p. 313-321.

Research output: Contribution to journalArticle

Wang, Yangming ; Silverman, Scott K. / A general two-step strategy to synthesize lariat RNAs. In: RNA. 2006 ; Vol. 12, No. 2. pp. 313-321.
@article{2b36a64351c448219a3de6afad789eb4,
title = "A general two-step strategy to synthesize lariat RNAs",
abstract = "We describe a general and efficient two-step strategy for lariat RNA synthesis. In the first step, a deoxyribozyme synthesizes 2′,5′- branched RNA. In the second step, T4 RNA ligase closes the loop that completes the lariat. The loop-closure reaction can form either a natural or unnatural lariat isomer, depending on which of the two 3′-termini of the branched RNA reacts with the lone 5′-end. We demonstrate two approaches to control formation of either lariat isomer. In conjunction with other routes for lariat RNA synthesis, the two-step strategy described here will facilitate biochemical studies that require lariat RNAs of varying nucleotide sequence. Published by Cold Spring Harbor Laboratory Press.",
keywords = "Branched RNA, Debranching enzyme, Deoxyribozyme, Lariat RNA, T4 RNA ligase",
author = "Yangming Wang and Silverman, {Scott K}",
year = "2006",
month = "2",
day = "1",
doi = "10.1261/rna.2259406",
language = "English (US)",
volume = "12",
pages = "313--321",
journal = "RNA",
issn = "1355-8382",
publisher = "Cold Spring Harbor Laboratory Press",
number = "2",

}

TY - JOUR

T1 - A general two-step strategy to synthesize lariat RNAs

AU - Wang, Yangming

AU - Silverman, Scott K

PY - 2006/2/1

Y1 - 2006/2/1

N2 - We describe a general and efficient two-step strategy for lariat RNA synthesis. In the first step, a deoxyribozyme synthesizes 2′,5′- branched RNA. In the second step, T4 RNA ligase closes the loop that completes the lariat. The loop-closure reaction can form either a natural or unnatural lariat isomer, depending on which of the two 3′-termini of the branched RNA reacts with the lone 5′-end. We demonstrate two approaches to control formation of either lariat isomer. In conjunction with other routes for lariat RNA synthesis, the two-step strategy described here will facilitate biochemical studies that require lariat RNAs of varying nucleotide sequence. Published by Cold Spring Harbor Laboratory Press.

AB - We describe a general and efficient two-step strategy for lariat RNA synthesis. In the first step, a deoxyribozyme synthesizes 2′,5′- branched RNA. In the second step, T4 RNA ligase closes the loop that completes the lariat. The loop-closure reaction can form either a natural or unnatural lariat isomer, depending on which of the two 3′-termini of the branched RNA reacts with the lone 5′-end. We demonstrate two approaches to control formation of either lariat isomer. In conjunction with other routes for lariat RNA synthesis, the two-step strategy described here will facilitate biochemical studies that require lariat RNAs of varying nucleotide sequence. Published by Cold Spring Harbor Laboratory Press.

KW - Branched RNA

KW - Debranching enzyme

KW - Deoxyribozyme

KW - Lariat RNA

KW - T4 RNA ligase

UR - http://www.scopus.com/inward/record.url?scp=31044439368&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=31044439368&partnerID=8YFLogxK

U2 - 10.1261/rna.2259406

DO - 10.1261/rna.2259406

M3 - Article

VL - 12

SP - 313

EP - 321

JO - RNA

JF - RNA

SN - 1355-8382

IS - 2

ER -