A General Strategy to Optimize the Performance of aza-BODIPY-based Probes for Enhanced Photoacoustic Properties

Anuj K. Yadav; Rodrigo Tapia Hernandez; Jefferson Chan

doi:10.1016/bs.mie.2021.06.022

A General Strategy to Optimize the Performance of aza-BODIPY-based Probes for Enhanced Photoacoustic Properties

Anuj K. Yadav
, Rodrigo Tapia Hernandez
, Jefferson Chan

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

In this chapter, we describe a generalizable strategy to obtain a high PA output platform that is optimized for ratiometric imaging. Our approach entails conformationally restricting pendant aryl rings on the aza-BODIPY core to enhance orbital overlap which consequently increases the extinction coefficient. This strategy can potentially be applied to other dye platforms to enhance their signal intensity. We provide detailed protocols for the synthesis, in vitro characterization, and in vivo application.

Original language	English (US)
Title of host publication	Photoacoustic Probes for In Vivo Imaging
Editors	Jefferson Chan
Publisher	Academic Press Inc.
Chapter	17
Pages	415-441
Number of pages	27
ISBN (Print)	978-0-323-85530-3
DOIs	https://doi.org/10.1016/bs.mie.2021.06.022
State	Published - 2021

Publication series

Name	Methods in Enzymology
Volume	657
ISSN (Print)	0076-6879
ISSN (Electronic)	1557-7988

Keywords

Boron Compounds
Spectrum Analysis
Conformational restriction
aza-BODIPY
Photoacoustic imaging
Hypoxia
Near infrared (NIR) region

ASJC Scopus subject areas

Molecular Biology
Biochemistry

Online availability

10.1016/bs.mie.2021.06.022

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Cite this

A General Strategy to Optimize the Performance of aza-BODIPY-based Probes for Enhanced Photoacoustic Properties. / Yadav, Anuj K.; Tapia Hernandez, Rodrigo; Chan, Jefferson.
Photoacoustic Probes for In Vivo Imaging. ed. / Jefferson Chan. Academic Press Inc., 2021. p. 415-441 (Methods in Enzymology; Vol. 657).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

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KW - Hypoxia

KW - Near infrared (NIR) region

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A General Strategy to Optimize the Performance of aza-BODIPY-based Probes for Enhanced Photoacoustic Properties

Abstract

Publication series

Keywords

ASJC Scopus subject areas

Online availability

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