A General Strategy to Optimize the Performance of aza-BODIPY-based Probes for Enhanced Photoacoustic Properties

Anuj K. Yadav, Rodrigo Tapia Hernandez, Jefferson Chan

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

In this chapter, we describe a generalizable strategy to obtain a high PA output platform that is optimized for ratiometric imaging. Our approach entails conformationally restricting pendant aryl rings on the aza-BODIPY core to enhance orbital overlap which consequently increases the extinction coefficient. This strategy can potentially be applied to other dye platforms to enhance their signal intensity. We provide detailed protocols for the synthesis, in vitro characterization, and in vivo application.

Original languageEnglish (US)
Title of host publicationPhotoacoustic Probes for In Vivo Imaging
EditorsJefferson Chan
PublisherAcademic Press Inc.
Chapter17
Pages415-441
Number of pages27
ISBN (Print)978-0-323-85530-3
DOIs
StatePublished - 2021

Publication series

NameMethods in Enzymology
Volume657
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Boron Compounds
  • Spectrum Analysis
  • Conformational restriction
  • aza-BODIPY
  • Photoacoustic imaging
  • Hypoxia
  • Near infrared (NIR) region

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

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