@article{3e51b4b751fc494f9070bb6482c32c6d,
title = "A General Approach to Convert Hemicyanine Dyes into Highly Optimized Photoacoustic Scaffolds for Analyte Sensing**",
abstract = "Most photoacoustic (PA) imaging agents are based on the repurposing of existing fluorescent dye platforms that exhibit non-optimal properties for PA applications. Herein, we introduce PA-HD, a new dye scaffold optimized for PA probe development that features a 4.8-fold increase in sensitivity and a red-shift of the λabs from 690 nm to 745 nm to enable ratiometric imaging. Computational modeling was used to elucidate the origin of these enhanced properties. To demonstrate the generalizability of our remodeling efforts, we developed three probes for β-galactosidase activity (PA-HD-Gal), nitroreductase activity (PA-HD-NTR), and H2O2 (PA-HD-H2O2). We generated two cancer models to evaluate PA-HD-Gal and PA-HD-NTR. We employed a murine model of Alzheimer's disease to test PA-HD-H2O2. There, we observed a PA signal increase at 735 nm of 1.79±0.20-fold relative to background, indicating the presence of oxidative stress. These results were confirmed via ratiometric calibration, which was not possible using the parent HD platform.",
keywords = "Alzheimer's disease, acoustogenic probe, analyte sensing, hydrogen peroxide, photoacoustic imaging",
author = "Gardner, {Sarah H.} and Brady, {Catharine J.} and Cameron Keeton and Yadav, {Anuj K.} and Mallojjala, {Sharath C.} and Lucero, {Melissa Y.} and Shengzhang Su and Zhengxin Yu and Hirschi, {Jennifer S.} and Mirica, {Liviu M.} and Jefferson Chan",
note = "Funding Information: The authors acknowledge the National Institutes of Health for support (R35GM133581 to J.C.; R01GM114588 to L.M.M.; R15GM142103‐01 to J.S.H.). S.C.M. acknowledges the supercomputing resources provided by XSEDE grant (TG‐CHE200009). C.J.B. acknowledges the Chemistry‐Biology Interface Training Grant (T32‐GM136629) and Robert C. and Carolyn J. Springborn Graduate Fellowship for support. M.Y.L. thanks the Alfred P. Sloan Foundation for financial support. Major funding for the 500 MHz Bruker CryoProbe was provided by the Roy J. Carver Charitable Trust (Muscatine, Iowa; Grant No. 15‐4521) to the School of Chemical Sciences NMR Lab. The Q‐Tof Ultima mass spectrometer was purchased in part with a grant from the National Science Foundation, Division of Biological Infrastructure (DBI‐0100085). We also acknowledge Dr. Iwona Dobrucka and the Molecular Imaging Laboratory at the Beckman Institute for use of the IVIS imaging system, Prof. Erik R. Nelson (Molecular and Integrative Physiology, UIUC) for ID8 and IGROV‐1 cells, Prof. Kai Zhang (Biochemistry, UIUC) for NeuroScreen‐1 cells, Sandy McMasters (UIUC Cell Media Facility) for help with preparation of cell culture media and technical expertise and Dr. Andrew Brannen (iThera Medical) for helpful suggestions. Funding Information: The authors acknowledge the National Institutes of Health for support (R35GM133581 to J.C.; R01GM114588 to L.M.M.; R15GM142103-01 to J.S.H.). S.C.M. acknowledges the supercomputing resources provided by XSEDE grant (TG-CHE200009). C.J.B. acknowledges the Chemistry-Biology Interface Training Grant (T32-GM136629) and Robert C. and Carolyn J. Springborn Graduate Fellowship for support. M.Y.L. thanks the Alfred P. Sloan Foundation for financial support. Major funding for the 500 MHz Bruker CryoProbe was provided by the Roy J. Carver Charitable Trust (Muscatine, Iowa; Grant No. 15-4521) to the School of Chemical Sciences NMR Lab. The Q-Tof Ultima mass spectrometer was purchased in part with a grant from the National Science Foundation, Division of Biological Infrastructure (DBI-0100085). We also acknowledge Dr. Iwona Dobrucka and the Molecular Imaging Laboratory at the Beckman Institute for use of the IVIS imaging system, Prof. Erik R. Nelson (Molecular and Integrative Physiology, UIUC) for ID8 and IGROV-1 cells, Prof. Kai Zhang (Biochemistry, UIUC) for NeuroScreen-1 cells, Sandy McMasters (UIUC Cell Media Facility) for help with preparation of cell culture media and technical expertise and Dr. Andrew Brannen (iThera Medical) for helpful suggestions. Publisher Copyright: {\textcopyright} 2021 Wiley-VCH GmbH",
year = "2021",
month = aug,
day = "16",
doi = "10.1002/anie.202105905",
language = "English (US)",
volume = "60",
pages = "18860--18866",
journal = "Angewandte Chemie - International Edition",
issn = "1433-7851",
publisher = "John Wiley & Sons, Ltd.",
number = "34",
}