Abstract

The endometrium is the lining of the uterus and site of blastocyst implantation. Each menstrual cycle, the endometrium cycles through rapid phases of growth, remodelling and breakdown. Significant vascular remodelling is also driven by trophoblast cells that form the outer layer of the blastocyst. Trophoblast invasion and remodelling enhance blood flow to the embryo ahead of placentation. Understanding the mechanisms of endometrial vascular remodelling and trophoblast invasion would provide key insights into endometrial physiology and cellular interactions critical for establishment of pregnancy. The objective of this study was to develop a tissue engineering platform to investigate the processes of endometrial angiogenesis and trophoblast invasion in a three-dimensional environment. We report adaptation of a methacrylamide-functionalized gelatin hydrogel that presents matrix stiffness in the range of the native tissue, supports the formation of endometrial endothelial cell networks with human umbilical vein endothelial cells and human endometrial stromal cells as an artificial endometrial perivascular niche and the culture of an endometrial epithelial cell layer, enables culture of a hormone-responsive stromal compartment and provides the capacity to monitor the kinetics of trophoblast invasion. With these studies, we provide a series of techniques that will instruct researchers in the development of endometrial models of increasing complexity.

Original languageEnglish (US)
Article number20190016
JournalInterface Focus
Volume9
Issue number5
DOIs
StatePublished - Oct 6 2019

Fingerprint

Hydrogel
Endothelial cells
Trophoblasts
Gelatin
Hydrogels
Hormones
Physiology
Stiffness matrix
Linings
Tissue engineering
Endometrium
Blood
Tissue
Kinetics
Placentation
Human Umbilical Vein Endothelial Cells
Blastocyst
Tissue Engineering
Menstrual Cycle
Stromal Cells

Keywords

  • Decidualization
  • Endometrium
  • Hydrogel
  • Stromal-endothelial
  • Trophoblast invasion

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

Cite this

A gelatin hydrogel to study endometrial angiogenesis and trophoblast invasion. / Zambuto, Samantha G.; Clancy, Kathryn B H; Harley, Brendan A.

In: Interface Focus, Vol. 9, No. 5, 20190016, 06.10.2019.

Research output: Contribution to journalArticle

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