A folic acid-based functionalized surface for biosensor systems

Kaustubh D. Bhalerao, Stephen C. Lee, Winston O. Soboyejo, Alfred B O Soboyejo

Research output: Contribution to journalArticle

Abstract

The performance of a biosensor depends largely on its interface with the biological system. This interface imparts a biologically relevant function to the device and provides a measure of specificity towards the biological analyte of interest. This paper documents the choice of folic acid as the functional component of a cantilever sensor to recognize nasopharyngeal (KB) cancer cells. A conjugation chemistry protocol has been outlined to deploy folic acid onto a titanium-coated sensor surface using a silane linker. The presence and biological activity of the sensor was verified by means of an immmunospecific (ELISA) procedure. The overall performance of the folic acid-based cantilever sensor was measured using cancerous KB cell-binding experiments.

Original languageEnglish (US)
Pages (from-to)3-8
Number of pages6
JournalJournal of Materials Science: Materials in Medicine
Volume18
Issue number1
DOIs
StatePublished - Jan 1 2007

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

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