The performance of a biosensor depends largely on its interface with the biological system. This interface imparts a biologically relevant function to the device and provides a measure of specificity towards the biological analyte of interest. This paper documents the choice of folic acid as the functional component of a cantilever sensor to recognize nasopharyngeal (KB) cancer cells. A conjugation chemistry protocol has been outlined to deploy folic acid onto a titanium-coated sensor surface using a silane linker. The presence and biological activity of the sensor was verified by means of an immmunospecific (ELISA) procedure. The overall performance of the folic acid-based cantilever sensor was measured using cancerous KB cell-binding experiments.
|Original language||English (US)|
|Number of pages||6|
|Journal||Journal of Materials Science: Materials in Medicine|
|State||Published - Jan 1 2007|
ASJC Scopus subject areas
- Biomedical Engineering