A first-generation porcine whole-genome radiation hybrid map

Rachel J. Hawken, Jeremy Murtaugh, Gail H. Flickinger, Martine Yerle, Anne Robic, Denis Milan, Joel Gellin, Craig W. Beattie, Lawrence B. Schook, Leeson J. Alexander

Research output: Contribution to journalArticlepeer-review

Abstract

A whole-genome radiation hybrid (WG-RH) panel was used to generate a first-generation radiation map of the porcine (Sus scrofa) genome. Over 900 Type I and II markers were used to amplify the INRA-University of Minnesota porcine Radiation Hybrid panel (IMpRH) comprised of 118 hybrid clones. Average marker retention frequency of 29.3% was calculated with 757 scorable markers. The RHMAP program established 128 linkage groups covering each chromosome (n = 19) at a lod ≥ 4.8. Fewer than 10% of the markers (59) could not be placed within any linkage group at a lod score ≥4.8. Linkage group order for each chromosome was determined by incorporating linkage data from the swine genetic map as well as physical assignments. The current map has an estimated ratio of ~70 kb/cR and a maximum theoretical resolution of 145 kb. This initial map forms a template for establishing accurate YAC and BAC contigs and eventual positional cloning of genes associated with complex traits.

Original languageEnglish (US)
Pages (from-to)824-830
Number of pages7
JournalMammalian Genome
Volume10
Issue number8
DOIs
StatePublished - Aug 26 1999
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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