A first-generation porcine whole-genome radiation hybrid map

Rachel J. Hawken; Jeremy Murtaugh; Gail H. Flickinger; Martine Yerle; Anne Robic; Denis Milan; Joel Gellin; Craig W. Beattie; Lawrence B. Schook; Leeson J. Alexander

doi:10.1007/s003359901097

A first-generation porcine whole-genome radiation hybrid map

Rachel J. Hawken, Jeremy Murtaugh, Gail H. Flickinger, Martine Yerle, Anne Robic, Denis Milan, Joel Gellin, Craig W. Beattie, Lawrence B. Schook, Leeson J. Alexander

Research output: Contribution to journal › Article › peer-review

Abstract

A whole-genome radiation hybrid (WG-RH) panel was used to generate a first-generation radiation map of the porcine (Sus scrofa) genome. Over 900 Type I and II markers were used to amplify the INRA-University of Minnesota porcine Radiation Hybrid panel (IMpRH) comprised of 118 hybrid clones. Average marker retention frequency of 29.3% was calculated with 757 scorable markers. The RHMAP program established 128 linkage groups covering each chromosome (n = 19) at a lod ≥ 4.8. Fewer than 10% of the markers (59) could not be placed within any linkage group at a lod score ≥4.8. Linkage group order for each chromosome was determined by incorporating linkage data from the swine genetic map as well as physical assignments. The current map has an estimated ratio of ~70 kb/cR and a maximum theoretical resolution of 145 kb. This initial map forms a template for establishing accurate YAC and BAC contigs and eventual positional cloning of genes associated with complex traits.

Original language	English (US)
Pages (from-to)	824-830
Number of pages	7
Journal	Mammalian Genome
Volume	10
Issue number	8
DOIs	https://doi.org/10.1007/s003359901097
State	Published - Aug 26 1999
Externally published	Yes

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Genetics

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title = "A first-generation porcine whole-genome radiation hybrid map",

abstract = "A whole-genome radiation hybrid (WG-RH) panel was used to generate a first-generation radiation map of the porcine (Sus scrofa) genome. Over 900 Type I and II markers were used to amplify the INRA-University of Minnesota porcine Radiation Hybrid panel (IMpRH) comprised of 118 hybrid clones. Average marker retention frequency of 29.3% was calculated with 757 scorable markers. The RHMAP program established 128 linkage groups covering each chromosome (n = 19) at a lod ≥ 4.8. Fewer than 10% of the markers (59) could not be placed within any linkage group at a lod score ≥4.8. Linkage group order for each chromosome was determined by incorporating linkage data from the swine genetic map as well as physical assignments. The current map has an estimated ratio of ~70 kb/cR and a maximum theoretical resolution of 145 kb. This initial map forms a template for establishing accurate YAC and BAC contigs and eventual positional cloning of genes associated with complex traits.",

author = "Hawken, {Rachel J.} and Jeremy Murtaugh and Flickinger, {Gail H.} and Martine Yerle and Anne Robic and Denis Milan and Joel Gellin and Beattie, {Craig W.} and Schook, {Lawrence B.} and Alexander, {Leeson J.}",

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AU - Hawken, Rachel J.

AU - Murtaugh, Jeremy

AU - Flickinger, Gail H.

AU - Yerle, Martine

AU - Robic, Anne

AU - Milan, Denis

AU - Gellin, Joel

AU - Beattie, Craig W.

AU - Schook, Lawrence B.

AU - Alexander, Leeson J.

PY - 1999/8/26

Y1 - 1999/8/26

N2 - A whole-genome radiation hybrid (WG-RH) panel was used to generate a first-generation radiation map of the porcine (Sus scrofa) genome. Over 900 Type I and II markers were used to amplify the INRA-University of Minnesota porcine Radiation Hybrid panel (IMpRH) comprised of 118 hybrid clones. Average marker retention frequency of 29.3% was calculated with 757 scorable markers. The RHMAP program established 128 linkage groups covering each chromosome (n = 19) at a lod ≥ 4.8. Fewer than 10% of the markers (59) could not be placed within any linkage group at a lod score ≥4.8. Linkage group order for each chromosome was determined by incorporating linkage data from the swine genetic map as well as physical assignments. The current map has an estimated ratio of ~70 kb/cR and a maximum theoretical resolution of 145 kb. This initial map forms a template for establishing accurate YAC and BAC contigs and eventual positional cloning of genes associated with complex traits.

AB - A whole-genome radiation hybrid (WG-RH) panel was used to generate a first-generation radiation map of the porcine (Sus scrofa) genome. Over 900 Type I and II markers were used to amplify the INRA-University of Minnesota porcine Radiation Hybrid panel (IMpRH) comprised of 118 hybrid clones. Average marker retention frequency of 29.3% was calculated with 757 scorable markers. The RHMAP program established 128 linkage groups covering each chromosome (n = 19) at a lod ≥ 4.8. Fewer than 10% of the markers (59) could not be placed within any linkage group at a lod score ≥4.8. Linkage group order for each chromosome was determined by incorporating linkage data from the swine genetic map as well as physical assignments. The current map has an estimated ratio of ~70 kb/cR and a maximum theoretical resolution of 145 kb. This initial map forms a template for establishing accurate YAC and BAC contigs and eventual positional cloning of genes associated with complex traits.

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A first-generation porcine whole-genome radiation hybrid map

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