A Dual-Beam Optical Microscope for Observation and Cleavage of Single DNA Molecules

William A. Lyon; Michelle M. Fang; William E. Haskins; Shuming Nie

doi:10.1021/ac980040+

A Dual-Beam Optical Microscope for Observation and Cleavage of Single DNA Molecules

William A. Lyon, Michelle M. Fang, William E. Haskins, Shuming Nie

Research output: Contribution to journal › Article › peer-review

Abstract

An integrated epifocal and evanescent-wave optical microscope has been developed for real-time observation and bond cleavage studies of single DNA molecules. Large genomic DNA is stretched in a laminar flow stream and is immobilized on a polylysine-coated glass surface by strong electrostatic interactions. Unlike previous single-molecule dynamics and single-enzyme studies, this work takes advantage of the elastic nature of double-stranded DNA and measures DNA relaxation events that are triggered by two phosphodiester (P-O) bond breaks. The ability to follow chemical reactions on individual DNA molecules opens new possibilities for DNA mapping and for studying DNA-protein interactions.

Original language	English (US)
Pages (from-to)	1743-1748
Number of pages	6
Journal	Analytical Chemistry
Volume	70
Issue number	9
DOIs	https://doi.org/10.1021/ac980040+
State	Published - May 1 1998
Externally published	Yes

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Analytical Chemistry

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10.1021/ac980040+

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AB - An integrated epifocal and evanescent-wave optical microscope has been developed for real-time observation and bond cleavage studies of single DNA molecules. Large genomic DNA is stretched in a laminar flow stream and is immobilized on a polylysine-coated glass surface by strong electrostatic interactions. Unlike previous single-molecule dynamics and single-enzyme studies, this work takes advantage of the elastic nature of double-stranded DNA and measures DNA relaxation events that are triggered by two phosphodiester (P-O) bond breaks. The ability to follow chemical reactions on individual DNA molecules opens new possibilities for DNA mapping and for studying DNA-protein interactions.

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A Dual-Beam Optical Microscope for Observation and Cleavage of Single DNA Molecules

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