Abstract
An integrated epifocal and evanescent-wave optical microscope has been developed for real-time observation and bond cleavage studies of single DNA molecules. Large genomic DNA is stretched in a laminar flow stream and is immobilized on a polylysine-coated glass surface by strong electrostatic interactions. Unlike previous single-molecule dynamics and single-enzyme studies, this work takes advantage of the elastic nature of double-stranded DNA and measures DNA relaxation events that are triggered by two phosphodiester (P-O) bond breaks. The ability to follow chemical reactions on individual DNA molecules opens new possibilities for DNA mapping and for studying DNA-protein interactions.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1743-1748 |
| Number of pages | 6 |
| Journal | Analytical Chemistry |
| Volume | 70 |
| Issue number | 9 |
| DOIs | |
| State | Published - May 1 1998 |
| Externally published | Yes |
ASJC Scopus subject areas
- Analytical Chemistry