@article{5afe3d74979f4af8b53e7b0a455b29a5,
title = "A distinct foliar pigmentation pattern formed by activator-repressor gradients upstream of an anthocyanin-activating R2R3-MYB",
abstract = "The emergence of novel traits is often preceded by a potentiation phase, when all the genetic components necessary for producing the trait are assembled. However, elucidating these potentiating factors is challenging. We have previously shown that an anthocyanin-activating R2R3-MYB, STRIPY, triggers the emergence of a distinct foliar pigmentation pattern in the monkeyflower Mimulus verbenaceus. Here, using forward and reverse genetics approaches, we identify three potentiating factors that pattern STRIPY expression: MvHY5, a master regulator of light signaling that activates STRIPY and is expressed throughout the leaf, and two leaf developmental regulators, MvALOG1 and MvTCP5, that are expressed in opposing gradients along the leaf proximodistal axis and negatively regulate STRIPY. These results provide strong empirical evidence that phenotypic novelties can be potentiated through incorporation into preexisting genetic regulatory networks and highlight the importance of positional information in patterning the novel foliar stripe.",
keywords = "anthocyanin, CP: Plants, evo-devo, Mimulus, pattern formation, phenotypic novelty, potentiation",
author = "LaFountain, {Amy M.} and Qiaoshan Lin and McMahon, {Hayley E.} and Ya Min and Baoqing Ding and Vandana Gurung and Seemann, {Jeffrey R.} and Yuan, {Yao Wu}",
note = "We thank three anonymous reviewers for constructive comments that improved our manuscript; Chang Liu, Bianca Ferreira, Amanda Garchow, Olivia Delello, Matt Opel, and Meghan Moriarty for exemplary plant care; Bianca Ferreira for preparing the illustrations used in the graphical abstract; Dr. Yun Zhou for providing the pGREEN800II-60 LUC plasmid; Dr. Nobutaka Mitsuda for providing the SRDX plasmid; and Dr. Tsuyoshi Nakagawa for providing the pGWB633 plasmid. This work was supported by the Ronald Bamford Fund Endowment for Ecology and Evolutionary Biology (to A.M.L.) and by an NIH grant (R01GM131055) (to Y.-W.Y.). A.M.L. J.S. and Y.-W.Y. designed the study. Experiments and analyses were performed by A.M.L. Q.L. H.E.M. Y.M. B.D. and V.G. A.M.L. and Y.-W.Y. wrote the manuscript, with input from all authors. The authors declare no competing interests. We thank three anonymous reviewers for constructive comments that improved our manuscript; Chang Liu, Bianca Ferreira, Amanda Garchow, Olivia Delello, Matt Opel, and Meghan Moriarty for exemplary plant care; Bianca Ferreira for preparing the illustrations used in the graphical abstract; Dr. Yun Zhou for providing the pGREEN800II-60 LUC plasmid; Dr. Nobutaka Mitsuda for providing the SRDX plasmid; and Dr. Tsuyoshi Nakagawa for providing the pGWB633 plasmid. This work was supported by the Ronald Bamford Fund Endowment for Ecology and Evolutionary Biology (to A.M.L.) and by an NIH grant ( R01GM131055 ) (to Y.-W.Y.).",
year = "2024",
month = jul,
day = "23",
doi = "10.1016/j.celrep.2024.114444",
language = "English (US)",
volume = "43",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "7",
}