A detection instrument for enhanced-fluorescence and label-free imaging on photonic crystal surfaces

Ian D. Block, Patrick C. Mathias, Nikhil Ganesh, Sarah I. Jones, Brian R. Dorvel, Vikram Chaudhery, Lila O. Vodkin, Rashid Bashir, Brian T. Cunningham

Research output: Contribution to journalArticle

Abstract

We report on the design and demonstration of an optical imaging system capable of exciting surface-bound fluorophores within the resonant evanescent electric field of a photonic crystal surface and gathering fluorescence emission that is directed toward the imaging objective by the photonic crystal. The system also has the ability to quantify shifts in the local resonance angle induced by the adsorption of biomolecules on the photonic crystal surface for label-free biomolecular imaging. With these two capabilities combined within a single detection system, we demonstrate label-free images self-registered to enhanced fluorescence images with 328x more sensitive fluorescence detection relative to a glass surface. This technique is applied to a DNA microarray where label-free quantification of immobilized capture DNA enables improved quality control and subsequent enhanced fluorescence detection of dye-tagged hybridized DNA yields 3x more genes to be detected versus commercially available microarray substrates.

Original languageEnglish (US)
Pages (from-to)13222-13235
Number of pages14
JournalOptics Express
Volume17
Issue number15
DOIs
StatePublished - Jul 20 2009

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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    Block, I. D., Mathias, P. C., Ganesh, N., Jones, S. I., Dorvel, B. R., Chaudhery, V., Vodkin, L. O., Bashir, R., & Cunningham, B. T. (2009). A detection instrument for enhanced-fluorescence and label-free imaging on photonic crystal surfaces. Optics Express, 17(15), 13222-13235. https://doi.org/10.1364/OE.17.013222