A dendritic single-molecule fluorescent probe that is monovalent, photostable and minimally blinking

Si Kyung Yang, Xinghua Shi, Seongjin Park, Taekjip Ha, Steven C. Zimmerman

Research output: Contribution to journalArticle

Abstract

Single-molecule fluorescence techniques have emerged as a powerful approach to understanding complex biological systems. However, a challenge researchers still face is the limited photostability of nearly all organic fluorophores, including the cyanine and Alexa dyes. We report a new, monovalent probe that emits in the far-red region of the visible spectrum with properties desirable for single-molecule optical imaging. This probe is based on a ring-fused boron-dipyrromethene (BODIPY) core that is conjugated to a polyglycerol dendrimer (PGD). The dendrimer makes the hydrophobic fluorophore water-soluble. This probe exhibits excellent brightness, with an emission maximum of 705 nm. We have observed strikingly long and stable emission from individual PGD-BODIPY probes, even in the absence of anti-fading agents such as Trolox, a combined oxidizing-reducing agent often used in single-molecule studies for improving the photostability of common imaging probes. These interesting properties greatly simplify use of the fluorophore.

Original languageEnglish (US)
Pages (from-to)692-697
Number of pages6
JournalNature Chemistry
Volume5
Issue number8
DOIs
StatePublished - Aug 1 2013

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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