A critical role of protein-bound water in the catalytic cycle of cytochrome P-450 camphor

Carmelo Di Primo, Stephen G. Sligar, Gaston Hui Bon Hoa, Pierre Douzou

Research output: Contribution to journalArticle

Abstract

The rates of NADH oxidation during the hydroxylation of camphor by cytochrome P-450cam were followed in the presence of co-solvents used to increase the osmotic pressure surrounding the protein-bound water. As a result, the measured Vmax decreases independently of the perturbant tested. Roughly 28 molecules of water, involved during the catalytic cycle, are deduced from the variation of Vmax as a function of osmotic pressure. These molecules, in part, could be those present in the cytochrome P.450cam-putidaredoxin interface.

Original languageEnglish (US)
Pages (from-to)252-254
Number of pages3
JournalFEBS Letters
Volume312
Issue number2-3
DOIs
StatePublished - Nov 9 1992

Keywords

  • Activity
  • Co-solvent
  • Cytochrome P-450
  • Osmotic pressure

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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