A coupled chlorinase-fluorinase system with a high efficiency of trans -halogenation and a shared substrate tolerance

H. Sun; H. Zhao; E. L. Ang

doi:10.1039/c8cc04436h

A coupled chlorinase-fluorinase system with a high efficiency of trans -halogenation and a shared substrate tolerance

H. Sun, H. Zhao, E. L. Ang

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Abstract

Enzymatic trans-halogenation enables radiolabeling under mild and aqueous conditions, but rapid reactions are desired. We developed a coupled chlorinase-fluorinase system for rapid trans-halogenation. Notably, the chlorinase shares a substrate tolerance with the fluorinase, enabling these two enzymes to cooperatively produce 5′-fluorodeoxy-2-ethynyladenosine (5′-FDEA) in up to 91.6% yield in 1 h.

Original language	English (US)
Pages (from-to)	9458-9461
Number of pages	4
Journal	Chemical Communications
Volume	54
Issue number	68
DOIs	https://doi.org/10.1039/c8cc04436h
State	Published - 2018

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/c8cc04436h

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abstract = "Enzymatic trans-halogenation enables radiolabeling under mild and aqueous conditions, but rapid reactions are desired. We developed a coupled chlorinase-fluorinase system for rapid trans-halogenation. Notably, the chlorinase shares a substrate tolerance with the fluorinase, enabling these two enzymes to cooperatively produce 5′-fluorodeoxy-2-ethynyladenosine (5′-FDEA) in up to 91.6% yield in 1 h.",

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AB - Enzymatic trans-halogenation enables radiolabeling under mild and aqueous conditions, but rapid reactions are desired. We developed a coupled chlorinase-fluorinase system for rapid trans-halogenation. Notably, the chlorinase shares a substrate tolerance with the fluorinase, enabling these two enzymes to cooperatively produce 5′-fluorodeoxy-2-ethynyladenosine (5′-FDEA) in up to 91.6% yield in 1 h.

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A coupled chlorinase-fluorinase system with a high efficiency of trans -halogenation and a shared substrate tolerance

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