A coupled chlorinase-fluorinase system with a high efficiency of trans -halogenation and a shared substrate tolerance

H. Sun, H. Zhao, E. L. Ang

Research output: Contribution to journalArticle

Abstract

Enzymatic trans-halogenation enables radiolabeling under mild and aqueous conditions, but rapid reactions are desired. We developed a coupled chlorinase-fluorinase system for rapid trans-halogenation. Notably, the chlorinase shares a substrate tolerance with the fluorinase, enabling these two enzymes to cooperatively produce 5′-fluorodeoxy-2-ethynyladenosine (5′-FDEA) in up to 91.6% yield in 1 h.

Original languageEnglish (US)
Pages (from-to)9458-9461
Number of pages4
JournalChemical Communications
Volume54
Issue number68
DOIs
StatePublished - Jan 1 2018

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ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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