A connected cytoskeleton network generates axonal tension in embryonic: Drosophila

Anthony Fan, Md Saddam Hossain Joy, M Taher A Saif

Research output: Contribution to journalArticle

Abstract

Axons of neurons are contractile, i.e., they actively maintain a rest tension. However, the spatial origin of this contractility along the axon and the role of the cytoskeleton in generating tension and sustaining rigidity are unknown. Here, using a microfluidic platform, we exposed a small segment of the axons of embryonic Drosophila motor neurons to specific cytoskeletal disruption drugs. We observed that a local actomyosin disruption led to a total loss in axonal tension, with the stiffness of the axon remaining unchanged. A local disruption of microtubules led to a local reduction in bending stiffness, while tension remained unchanged. These observations demonstrated that contractile forces are generated and transferred along the entire length of the axon in a serial fashion. Thus, a local force disruption results in a collapse of tension of the entire axon. This mechanism potentially provides a pathway for rapid tension regulation to facilitate physiological processes that are influenced by axonal tension.

Original languageEnglish (US)
Pages (from-to)3133-3139
Number of pages7
JournalLab on a chip
Volume19
Issue number18
DOIs
StatePublished - Jan 1 2019

Fingerprint

Cytoskeleton
Drosophila
Axons
Neurons
Stiffness
Physiological Phenomena
Actomyosin
Microfluidics
Motor Neurons
Microtubules
Rigidity
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

A connected cytoskeleton network generates axonal tension in embryonic : Drosophila. / Fan, Anthony; Joy, Md Saddam Hossain; Saif, M Taher A.

In: Lab on a chip, Vol. 19, No. 18, 01.01.2019, p. 3133-3139.

Research output: Contribution to journalArticle

Fan, Anthony ; Joy, Md Saddam Hossain ; Saif, M Taher A. / A connected cytoskeleton network generates axonal tension in embryonic : Drosophila. In: Lab on a chip. 2019 ; Vol. 19, No. 18. pp. 3133-3139.
@article{387ace218fdc4bacb57eb19c8a27923a,
title = "A connected cytoskeleton network generates axonal tension in embryonic: Drosophila",
abstract = "Axons of neurons are contractile, i.e., they actively maintain a rest tension. However, the spatial origin of this contractility along the axon and the role of the cytoskeleton in generating tension and sustaining rigidity are unknown. Here, using a microfluidic platform, we exposed a small segment of the axons of embryonic Drosophila motor neurons to specific cytoskeletal disruption drugs. We observed that a local actomyosin disruption led to a total loss in axonal tension, with the stiffness of the axon remaining unchanged. A local disruption of microtubules led to a local reduction in bending stiffness, while tension remained unchanged. These observations demonstrated that contractile forces are generated and transferred along the entire length of the axon in a serial fashion. Thus, a local force disruption results in a collapse of tension of the entire axon. This mechanism potentially provides a pathway for rapid tension regulation to facilitate physiological processes that are influenced by axonal tension.",
author = "Anthony Fan and Joy, {Md Saddam Hossain} and Saif, {M Taher A}",
year = "2019",
month = "1",
day = "1",
doi = "10.1039/c9lc00243j",
language = "English (US)",
volume = "19",
pages = "3133--3139",
journal = "Lab on a Chip - Miniaturisation for Chemistry and Biology",
issn = "1473-0197",
publisher = "Royal Society of Chemistry",
number = "18",

}

TY - JOUR

T1 - A connected cytoskeleton network generates axonal tension in embryonic

T2 - Drosophila

AU - Fan, Anthony

AU - Joy, Md Saddam Hossain

AU - Saif, M Taher A

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Axons of neurons are contractile, i.e., they actively maintain a rest tension. However, the spatial origin of this contractility along the axon and the role of the cytoskeleton in generating tension and sustaining rigidity are unknown. Here, using a microfluidic platform, we exposed a small segment of the axons of embryonic Drosophila motor neurons to specific cytoskeletal disruption drugs. We observed that a local actomyosin disruption led to a total loss in axonal tension, with the stiffness of the axon remaining unchanged. A local disruption of microtubules led to a local reduction in bending stiffness, while tension remained unchanged. These observations demonstrated that contractile forces are generated and transferred along the entire length of the axon in a serial fashion. Thus, a local force disruption results in a collapse of tension of the entire axon. This mechanism potentially provides a pathway for rapid tension regulation to facilitate physiological processes that are influenced by axonal tension.

AB - Axons of neurons are contractile, i.e., they actively maintain a rest tension. However, the spatial origin of this contractility along the axon and the role of the cytoskeleton in generating tension and sustaining rigidity are unknown. Here, using a microfluidic platform, we exposed a small segment of the axons of embryonic Drosophila motor neurons to specific cytoskeletal disruption drugs. We observed that a local actomyosin disruption led to a total loss in axonal tension, with the stiffness of the axon remaining unchanged. A local disruption of microtubules led to a local reduction in bending stiffness, while tension remained unchanged. These observations demonstrated that contractile forces are generated and transferred along the entire length of the axon in a serial fashion. Thus, a local force disruption results in a collapse of tension of the entire axon. This mechanism potentially provides a pathway for rapid tension regulation to facilitate physiological processes that are influenced by axonal tension.

UR - http://www.scopus.com/inward/record.url?scp=85071998442&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85071998442&partnerID=8YFLogxK

U2 - 10.1039/c9lc00243j

DO - 10.1039/c9lc00243j

M3 - Article

C2 - 31435630

AN - SCOPUS:85071998442

VL - 19

SP - 3133

EP - 3139

JO - Lab on a Chip - Miniaturisation for Chemistry and Biology

JF - Lab on a Chip - Miniaturisation for Chemistry and Biology

SN - 1473-0197

IS - 18

ER -