A conditionally lethal yeast mutant blocked at the first step in glycosyl phosphatidylinositol anchor synthesis

Steven D. Leidich, Darren A. Drapp, Peter Orlean

Research output: Contribution to journalArticlepeer-review

Abstract

Glycosyl phosphatidylinositols (GPIs) anchor many proteins to the surface of eukaryotic cells and may also serve as sorting signals on proteins and participate in signal transduction. We have isolated a Saccharomyces cerevisiae GPI anchoring mutant, gpi1, using a colony screen for cells blocked in [3H]inositol incorporation into protein. The gpi1 mutant is defective in vitro in the synthesis of N-acetylglucosaminyl phosphatidylinositol, the first intermediate in GPI synthesis, and is also temperature-sensitive for growth. Completion of the first step in GPI assembly is therefore required for growth of the unicellular eukaryote S. cerevisiae. GPI synthesis could therefore be exploited as a target for antifungal or antiparasitic agents.

Original languageEnglish (US)
Pages (from-to)10193-10196
Number of pages4
JournalJournal of Biological Chemistry
Volume269
Issue number14
StatePublished - Apr 8 1994

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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