TY - JOUR
T1 - A comprehensive temporal patterning gene network in Drosophila medulla neuroblasts revealed by single-cell RNA sequencing
AU - Zhu, Hailun
AU - Zhao, Sihai Dave
AU - Ray, Alokananda
AU - Zhang, Yu
AU - Li, Xin
N1 - We thank the Flow Cytometry Facility and the DNA Services Laboratory, and the High-Performance Computing in Biology Group of the Roy J. Carver Biotechnology Center at the University of Illinois at Urbana-Champaign for FACS sorting, and for construction and sequencing of the 10x V3 Single-Cell libraries, and initial analysis of the sequencing data, respectively, and for providing the corresponding method part. We thank the fly community, especially Claude Desplan, Steven Russell, Cheng-Yu Lee, Tzumin Lee, Deborah Hursh, J. Peter Gergen, Tiffany Cook, Louise Cheng, Ruth Lehmann, Adrian Moore, Chris Doe; Richard Mann, Andrew Tomlinson, John R. Nambu, Makoto Sato, and Tetsuya Kojima, for generous gifts of antibodies and fly stocks. We thank the Bloomington Drosophila Stock Center, FlyORF, the Vienna Drosophila RNAi Center, the Developmental Studies Hybridoma Bank, and TriP at Harvard Medical School (NIH/NIGMS R01-GM084947) for fly stocks and reagents. We would like to thank the NSF-Simons Center for Quantitative Biology at Northwestern University for supporting this project as a Pilot grant, and also the National Eye Institute for grant support (Grant 1 R01 EY026965-01A1 to XL).
We thank the Flow Cytometry Facility and the DNA Services Laboratory, and the High-Performance Computing in Biology Group of the Roy J. Carver Biotechnology Center at the University of Illinois at Urbana-Champaign for FACS sorting, and for construction and sequencing of the 10x V3 Single-Cell libraries, and initial analysis of the sequencing data, respectively, and for providing the corresponding method part. We thank the fly community, especially Claude Desplan, Steven Russell, Cheng-Yu Lee, Tzumin Lee, Deborah Hursh, J. Peter Gergen, Tiffany Cook, Louise Cheng, Ruth Lehmann, Adrian Moore, Chris Doe; Richard Mann, Andrew Tomlinson, John R. Nambu, Makoto Sato, and Tetsuya Kojima, for generous gifts of antibodies and fly stocks. We thank the Bloomington Drosophila Stock Center, FlyORF, the Vienna Drosophila RNAi Center, the Developmental Studies Hybridoma Bank, and TriP at Harvard Medical School (NIH/NIGMS R01-GM084947) for fly stocks and reagents. We would like to thank the NSF-Simons Center for Quantitative Biology at Northwestern University for supporting this project as a Pilot grant, and also the National Eye Institute for grant support (Grant 1 R01 EY026965-01A1 to XL).
PY - 2022/12
Y1 - 2022/12
N2 - During development, neural progenitors are temporally patterned to sequentially generate a variety of neural types. In Drosophila neural progenitors called neuroblasts, temporal patterning is regulated by cascades of Temporal Transcription Factors (TTFs). However, known TTFs were mostly identified through candidate approaches and may not be complete. In addition, many fundamental questions remain concerning the TTF cascade initiation, progression, and termination. In this work, we use single-cell RNA sequencing of Drosophila medulla neuroblasts of all ages to identify a list of previously unknown TTFs, and experimentally characterize their roles in temporal patterning and neuronal specification. Our study reveals a comprehensive temporal gene network that patterns medulla neuroblasts from start to end. Furthermore, the speed of the cascade progression is regulated by Lola transcription factors expressed in all medulla neuroblasts. Our comprehensive study of the medulla neuroblast temporal cascade illustrates mechanisms that may be conserved in the temporal patterning of neural progenitors.
AB - During development, neural progenitors are temporally patterned to sequentially generate a variety of neural types. In Drosophila neural progenitors called neuroblasts, temporal patterning is regulated by cascades of Temporal Transcription Factors (TTFs). However, known TTFs were mostly identified through candidate approaches and may not be complete. In addition, many fundamental questions remain concerning the TTF cascade initiation, progression, and termination. In this work, we use single-cell RNA sequencing of Drosophila medulla neuroblasts of all ages to identify a list of previously unknown TTFs, and experimentally characterize their roles in temporal patterning and neuronal specification. Our study reveals a comprehensive temporal gene network that patterns medulla neuroblasts from start to end. Furthermore, the speed of the cascade progression is regulated by Lola transcription factors expressed in all medulla neuroblasts. Our comprehensive study of the medulla neuroblast temporal cascade illustrates mechanisms that may be conserved in the temporal patterning of neural progenitors.
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UR - http://www.scopus.com/inward/citedby.url?scp=85126260891&partnerID=8YFLogxK
U2 - 10.1038/s41467-022-28915-3
DO - 10.1038/s41467-022-28915-3
M3 - Article
C2 - 35273186
AN - SCOPUS:85126260891
SN - 2041-1723
VL - 13
JO - Nature communications
JF - Nature communications
IS - 1
M1 - 1247
ER -