A comparison of anionic sites in the glomerular basement membranes from different classes of fishes

Robert B. Boyd, Arthur L. DeVries

Research output: Contribution to journalArticlepeer-review

Abstract

Cationized ferritin was injected into the circulatory system of teleosts, the sea raven and Atlantic eelpout, and into elasmobranchs, the spiny dogfish and the skate, to determine if the glomerular basement membranes (GBM) from these different groups of fishes possess anionic binding sites similar to those present in the GBM of mammals. The distribution of cationized ferritin was the same in all fishes listed. Cationized ferritin was localized only in the GBM and the mesangial matrix. The regular distribution of cationized ferritin within the laminae rarae (∼60 nm intervals) was taken as evidence of the presence of anionic binding sites. Cationized ferritin did not bind to the glomerular capillary endothelium, nor was any of it localized at the base of the slit diaphragms of the foot processes of the podocytes. The distribution of binding sites in the GBM of these fishes is similar to that in another teleost, the winter flounder, and in a cyclostome, the hagfish.

Original languageEnglish (US)
Pages (from-to)513-517
Number of pages5
JournalCell and Tissue Research
Volume245
Issue number3
DOIs
StatePublished - Sep 1 1986

Keywords

  • Anionic binding sites
  • Cationized ferritin
  • Elasmobranchs
  • Glomerular basement membrane
  • Permeability
  • Teleosts

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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