A comparative chlorophyll a fluorescence study on isolated cells and intacleaves of bouteloua gracilis (Blue grama grass)

B. Jiménez-Francisco, A. Stirbet, G. A. Aguado-Santacruz, H. Campos, F. V. Conde-Martínez, D. Padilla-Chacón, C. Trejo, C. J. Bernacchi, G. Govindjee

Research output: Contribution to journalArticlepeer-review

Abstract

Bouteloua gracilis (blue grama grass) is one of the most drought and grazing tolerant plants in the short-grass ecosystem. To obtain information on their photosystem activities, we measured the fast (< 1 s) chlorophyll a fluorescence transient (the OJIP curve) from their leaves, and isolated cells grown photoautotrophically in suspension in a culture medium, or with added sucrose. One of our goals was to study the effect of different sucrose concentrations (0, 0.15, 0.3, and 3%) on PSII activity in isolated cells. Our results on cells suspended in culture medium, using the JIP-test, showed a decrease in PSII activity at increasing sucrose concentrations, while the photoautotrophic cells showed an optimal PSII activity, close to that of the leaves. Further, our data on cells grown in 0, 0.15, and 0.3% sucrose, but with added CO2, and measured while the cells were deposited on filter paper, indicate that supplementary CO2 can increase the PSII activity in the presence of sucrose, although further research is necessary to understand these results.

Original languageEnglish (US)
Pages (from-to)262-274
Number of pages13
JournalPhotosynthetica
Volume58
Issue numberSpecial Issue
DOIs
StatePublished - 2020

Keywords

  • Kinetic parameters of the O-J, J-I, and I-P phases
  • Osmotic stress
  • Performance index

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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