TY - JOUR
T1 - A comparative chlorophyll a fluorescence study on isolated cells and intacleaves of bouteloua gracilis (Blue grama grass)
AU - Jiménez-Francisco, B.
AU - Stirbet, A.
AU - Aguado-Santacruz, G. A.
AU - Campos, H.
AU - Conde-Martínez, F. V.
AU - Padilla-Chacón, D.
AU - Trejo, C.
AU - Bernacchi, C. J.
AU - Govindjee, G.
N1 - Funding Information:
Abbreviations: Area (as related to chlorophyll a fluorescence induction curve) – area between the OJIP transient and the horizontal line at maximum fluorescence, FM; Chl – chlorophyll; ChlFI – chlorophyll a fluorescence induction; DCMU (diuron) – 3-(3,4-dichlorophenyl)-1,1-dimethylurea; FK, FJ (fluorescence at the J level), FI (fluorescence at the I level), and FP (fluorescence at the P level) – chlorophyll a fluorescence at 0.3, 2, 30 ms, and at the peak P of chlorophyll a fluorescence induction, respectively; FM – maximum Chl a fluorescence; FO – minimum Chl a fluorescence (fluorescence at the O level); FV – the (maximum) variable fluorescence, which is FM – FO; OEC – oxygen-evolving complex; OJIP transient – the Chl a fluorescence transient from FO to FP; PQ – plastoquinone; PSI – photosystem I; PSII – photosystem II; QA and QB – primary and secondary plastoquinone electron acceptors of the photosystem II. Acknowledgements: Govindjee thanks the staff of Information Technology in Life Sciences at the University of Illinois at Urbana-Champaign (UIUC) for their help with the use of computers; he is also grateful to the staff of the Department of Plant Biology and the Department of Biochemistry at UIUC for their support. This research was initiated in the Laboratory of Biophysics & Environmental Plant Physiology, Department of Botany, Postgraduate College, Montecillo, Mexico. Betza Jimenez-Francisco thanks the National Council of Science and Technology (CONACYT), Mexico, for a Ph.D. fellowship and financial support for a visit to the laboratories of Govindjee and Carl Bernacchi, at UIUC, for training and experiments. †The authors contributed equally. aSince 2019, Govindjee (who had earlier published under one name only) is publishing under his new formal name: Govindjee Govindjee. This article honors Reto J. Strasser at his 75th birthday for his outstanding and unique contributions in the use and analysis of chlorophyll a fluorescence towards understanding photosynthesis; see also Govindjee et al. (2019).
Funding Information:
Govindjee thanks the staff of Information Technology in Life Sciences at the University of Illinois at Urbana-Champaign (UIUC) for their help with the use of computers; he is also grateful to the staff of the Department of Plant Biology and the Department of Biochemistry at UIUC for their support. This research was initiated in the Laboratory of Biophysics & Environmental Plant Physiology, Department of Botany, Postgraduate College, Montecillo, Mexico. Betza Jimenez-Francisco thanks the National Council of Science and Technology (CONACYT), Mexico, for a Ph.D. fellowship and financial support for a visit to the laboratories of Govindjee and Carl Bernacchi, at UIUC, for training and experiments.
Publisher Copyright:
© The authors.
PY - 2020
Y1 - 2020
N2 - Bouteloua gracilis (blue grama grass) is one of the most drought and grazing tolerant plants in the short-grass ecosystem. To obtain information on their photosystem activities, we measured the fast (< 1 s) chlorophyll a fluorescence transient (the OJIP curve) from their leaves, and isolated cells grown photoautotrophically in suspension in a culture medium, or with added sucrose. One of our goals was to study the effect of different sucrose concentrations (0, 0.15, 0.3, and 3%) on PSII activity in isolated cells. Our results on cells suspended in culture medium, using the JIP-test, showed a decrease in PSII activity at increasing sucrose concentrations, while the photoautotrophic cells showed an optimal PSII activity, close to that of the leaves. Further, our data on cells grown in 0, 0.15, and 0.3% sucrose, but with added CO2, and measured while the cells were deposited on filter paper, indicate that supplementary CO2 can increase the PSII activity in the presence of sucrose, although further research is necessary to understand these results.
AB - Bouteloua gracilis (blue grama grass) is one of the most drought and grazing tolerant plants in the short-grass ecosystem. To obtain information on their photosystem activities, we measured the fast (< 1 s) chlorophyll a fluorescence transient (the OJIP curve) from their leaves, and isolated cells grown photoautotrophically in suspension in a culture medium, or with added sucrose. One of our goals was to study the effect of different sucrose concentrations (0, 0.15, 0.3, and 3%) on PSII activity in isolated cells. Our results on cells suspended in culture medium, using the JIP-test, showed a decrease in PSII activity at increasing sucrose concentrations, while the photoautotrophic cells showed an optimal PSII activity, close to that of the leaves. Further, our data on cells grown in 0, 0.15, and 0.3% sucrose, but with added CO2, and measured while the cells were deposited on filter paper, indicate that supplementary CO2 can increase the PSII activity in the presence of sucrose, although further research is necessary to understand these results.
KW - Kinetic parameters of the O-J, J-I, and I-P phases
KW - Osmotic stress
KW - Performance index
UR - http://www.scopus.com/inward/record.url?scp=85085396343&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85085396343&partnerID=8YFLogxK
U2 - 10.32615/ps.2019.148
DO - 10.32615/ps.2019.148
M3 - Article
AN - SCOPUS:85085396343
SN - 0300-3604
VL - 58
SP - 262
EP - 274
JO - Photosynthetica
JF - Photosynthetica
IS - Special Issue
ER -